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Anti-Human CD133 (GC4) h(CD28-41BB-CD3Z) CAR Lentivirus Packaging Kit (XS-0622-YF127)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

This kit provides a complete lentivirus packaging system to produce anti-CD133 (GC4) h(4-1BB-CD3ζ) CAR lentivirus, which enables CAR-T cells generation to target Human CD133. It contains plasmids that express all the viral proteins necessary, and CAR detection reagent, providing a full set of reagents for CAR lentivirus packaging and CAR expression evaluation. The expressed anti-CD133 CAR lentiviruses are HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells. These viruses constitutively express the scFv portion of anti-CD133 (GC4) linked to 3nd generation CAR containing CD28, 41BB and CD3ζ signaling domains. CAR detection reagent can be used to detect the positive expression rate of CAR-T cells after lentiviral transduction.

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Description

  • Target
  • CD133
  • Components
  • This kit contains a CAR lentiviral vector, packaging vectors, CAR detection reagent, and 293 cells.
    1. A CAR lentiviral vector
    • Anti-CD133 (GC4) h(CD28-41BB-CD3ζ) CAR
    2. Three packaging vectors
    • LentiArt™ pHelp1
    • LentiArt™ pHelp2
    • LentiArt™ pHelp3
    3. CAR detection reagent
    4. 293 cells
  • Size
  • 10 reactions; 20 reactions; 50 reactions
  • Storage Conditions
  • For details of the storage requirements, please see the User Manual for instructions.
  • Applications
  • CAR lentivirus packaging; CAR lentivirus transfection; CAR-T cell development; CAR-T cell therapy.

CAR Vector

  • CAR Generation
  • 3nd-generation CAR
  • Vector Name
  • pCDCAR1
  • Vector Length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Receptor Construction
  • scFv-CD28-41BB-CD3ζ

Target

  • Gene Name
  • PROM1
  • Full Name
  • prominin 1
  • scFv
  • GC4
  • Host Species
  • Human
  • Target Species
  • Human
  • Target Background
  • This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.
  • Synonyms
  • PROM1; prominin 1; RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061; prominin-1; hProminin; antigen AC133
  • Related Disease
  • Pancreatic cancer, Colorectal cancer

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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