Anti-Human CD22 (Inotuzumab) h(CD28-41BB-CD3Z) CAR Lentivirus Packaging Kit (XS-0622-YF133)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

This kit provides a complete lentivirus packaging system to produce anti-CD22 (Inotuzumab) h(4-1BB-CD3ζ) CAR lentivirus, which enables CAR-T cells generation to target Human CD22. It contains plasmids that express all the viral proteins necessary, and CAR detection reagent, providing a full set of reagents for CAR lentivirus packaging and CAR expression evaluation. The expressed anti-CD22 CAR lentiviruses are HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells. These viruses constitutively express the scFv portion of anti-CD22 (Inotuzumab) linked to 3nd generation CAR containing CD28, 41BB and CD3ζ signaling domains. CAR detection reagent can be used to detect the positive expression rate of CAR-T cells after lentiviral transduction.

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Description

Target
CD22

Components
This kit contains a CAR lentiviral vector, packaging vectors, CAR detection reagent, and 293 cells.
1. A CAR lentiviral vector
• Anti-CD22 (Inotuzumab) h(CD28-41BB-CD3ζ) CAR
2. Three packaging vectors
• LentiArt™ pHelp1
• LentiArt™ pHelp2
• LentiArt™ pHelp3
3. CAR detection reagent
4. 293 cells

Size
10 reactions; 20 reactions; 50 reactions

Storage Conditions
For details of the storage requirements, please see the User Manual for instructions.

Applications
CAR lentivirus packaging; CAR lentivirus transfection; CAR-T cell development; CAR-T cell therapy.

CAR Vector

CAR Generation
3nd-generation CAR

Vector Name
pCDCAR1

Vector Length
~8kb

Vector Type
Lentiviral vector

Receptor Construction
scFv-CD28-41BB-CD3ζ

Target

Gene Name
CD22

Full Name
CD22 Molecule

scFv
Inotuzumab

Host Species
Humanized

Target Species
Human

Gene ID
933

Uniprot ID
P20273

Target Background
Predicted to enable CD4 receptor binding activity; protein phosphatase binding activity; and sialic acid binding activity. Involved in B cell activation; negative regulation of B cell receptor signaling pathway; and regulation of endocytosis. Located in early endosome and recycling endosome.

Synonyms
CD22 Molecule; CD22 Antigen; Sialic Acid-Binding Ig-Like Lectin 2; B-Lymphocyte Cell Adhesion Molecule; T-Cell Surface Antigen Leu-14; SIGLEC-2

Related Disease
Recurrent or Refractory B Cell Acute Lymphoblastic Leukemia

Related Resources

Overview of CD22-Targeted CAR Cell Therapies

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.