Anti-Human HER2 (HER2-1) TCR (HLA-A*0201/KIFGSLAFL) Lentivirus Packaging Kit (XS-0622-YF181)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

This kit provides a complete lentivirus packaging system to produce anti-HER2 (HER2-1) TCR lentivirus, which enables TCR-T cells generation to target HER2. It contains plasmids that express all the viral proteins necessary, and TCR detection reagent, providing a full set of reagents for TCR lentivirus packaging and TCR expression evaluation. The expressed anti-HER2 TCR lentiviruses are HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells. These viruses constitutively express the TCR restricted to HLA-A*0201/KIFGSLAFL(369-377). TCR detection reagent can be used to detect the positive expression rate of TCR-T cells after lentiviral transduction.

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Description

Target
HER2

Components
This kit contains a TCR lentiviral vector, packaging vectors, TCR detection reagent, and 293 cells.
1. A TCR lentiviral vector
• Anti-HER2 (HER2-1) TCR plasmid
2. Three packaging vectors
• LentiArt™ pHelp1
• LentiArt™ pHelp2
• LentiArt™ pHelp3
3. TCR detection reagent
4. 293 cells

Size
10 reactions; 20 reactions; 50 reactions

Storage Conditions
For details of the storage requirements, please see the User Manual for instructions.

Applications
TCR lentivirus packaging; TCR lentivirus transfection; TCR-T cell development; TCR-T cell therapy.

TCR Vector

MHC Allele
HLA-A*0201

Peptide
KIFGSLAFL

Epitope
369-377

Vector Name
pCDCAR1

Vector Length
~8kb

Vector Type
Lentiviral vector

TCR Construction
TCR alpha-P2A-TCR beta

Target

Gene Name
ERBB2

Full Name
erb-b2 receptor tyrosine kinase 2

scFv
HER2-1

Host Species
Human

Target Species
Human

Gene ID
2064

Uniprot ID
P04626

Target Background
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Synonyms
NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Related Disease
Breast cancer

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.