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Anti-IL11Rα (18A11)-CD147 CAR-MA (CARMA-W1003)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The vector of anti-IL11Rα chimeric antigen receptor (CAR) is constructed for the engineering of macrophage cells to target human IL11Rα and induce target cell phagocytosis. The macrophage cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-IL11Rα antibody (18A11) linked to CD147 signaling domains. The vector product was designed for the treatment of Osteosarcoma.

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Details

  • Target
  • IL11Rα
  • Cell Type
  • Macrophage
  • Targeting Diseases
  • Osteosarcoma
  • Vector Name
  • pCDCAR1
  • Vector length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Size
  • 10 µg
  • Storage
  • Store at -20°C for long term.

CAR Components

  • Promoter
  • EF1a
  • scFv
  • 18A11
  • Hinge
  • IgG1
  • TM
  • CD147
  • ICD
  • CD147
  • Marker
  • GFP or N/A
  • Receptor Construction
  • scFv-hinge(IgG1)-TM(CD147)-ICD(CD147)
  • Discription of Signaling Cassetes
  • To breakdown the "physical barrier" of the tumour matrix, CD147 is used as the signaling domain. It's a membrane molecule that is essential for ECM remodelling via the expression of MMPs.

Target

  • Target
  • IL11Rα
  • Target Species
  • Human
  • Clone
  • 18A11
  • Host
  • Mouse
  • Gene Name
  • Interleukin 11 receptor subunit alpha
  • Introduction
  • Interleukin 11 is a stromal cell-derived cytokine that belongs to a family of pleiotropic and redundant cytokines that use the gp130 transducing subunit in their high affinity receptors. This gene encodes the IL-11 receptor, which is a member of the hematopoietic cytokine receptor family. This particular receptor is very similar to ciliary neurotrophic factor, since both contain an extracellular region with a 2-domain structure composed of an immunoglobulin-like domain and a cytokine receptor-like domain. Multiple alternatively spliced transcript variants have been found for this gene.
  • Alternative Names
  • CRSDA; IL11RA; IL11Rα; Interleukin 11 receptor subunit alpha

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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