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Anti-L1CAM (AB417) h(CD28-CD3ζ) CAR-T Cells [Humanized Mouse (CD34+ HSC)] (MCAR-P325)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Adoptive immunotherapy using T cells expressing chimeric antigen receptors (CARs) has produced remarkable clinical outcomes. However, much of the mechanisms of action, such as the development of memory responses and sources of immune cytokines, remain elusive largely due to the challenge of characterizing human CAR T cell function in vivo. Creative Biolabs has established a humanized mouse (hu-mouse) model with a functional human immune system and genetically-matched (autologous) Ovarian cancer that permits modeling of L1CAM-targeted CAR T cell therapy in immunocompetent hosts without allogeneic or xenogeneic immune responses. Our anti-L1CAM CAR T cells are prepared from freshly isolated T lymphocytes in a syngeneic mouse model, followed by ex vivo transduction with anti-L1CAM CAR lentiviral particles and amplification process to generate CAR T cells. These humanized CD34+ mice-derived CAR-T cells offer many advantages including permitting the evaluation of antitumor responses in immunocompetent NSG hosts, testing human CAR-T constructs against human primary malignance, and preclinical evaluation of CAR T cell therapies.

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T Cell Background

  • Target
  • L1CAM
  • Applications
  • The humanized L1CAM CAR-T cells can be used for evaluation of the safety, toxicity, persistence and exhaustion of CAR T cells in a humanized CD34+ mouse model.
  • T Cell Source
  • Humanized CD34+ HSC Mouse Model
  • Donor Mouse Strain
  • BALB/c
  • Donor Tumor Cell Line
  • Ovarian cancer Cell Line
  • Mouse Modeling
  • Humanized CD34+ mice is a study-ready mouse model with a human-like immune system, created by adoptive transfer of CD34+ stem cells. Humanized CD34+ mice are supreme in vivo models to study immunology and immuno-oncology.

    Our Hu-CD34+ mice produced by injecting CD34+ hematopoietic stem cells (hu-CD34) demonstrate robust multi-lineage engraftment of human immune cell populations including very good T cell maturation and function for long-term studies. Successful humanization of each mouse is quantified from mouse peripheral blood via flow cytometry using anti- hu-CD45+ and anti-murine CD45+. The presence of human immune cells are verified in the peripheral blood as early as 12-15 weeks post-engraftment, as verified by multi-color flow cytometry. These immune cells are detectable in mouse tissues, including bone marrow, spleen, liver, and lung, and have been shown to infiltrate implanted tumors.
  • T Cell Isolation
  • The human T cells were isolated from hu-mouse spleens by MACS using anti-human CD3 micro-beads.

CAR Design

  • CAR Introduction
  • The vector of anti-L1CAM chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target Human L1CAM. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-L1CAM antibody linked to CD28 and CD3ζ signaling domains. And the vector product was designed for the treatment of Ovarian cancer.
  • scFv Clone
  • AB417
  • Target Species
  • Human
  • scFv Host Species
  • Human
  • CAR Construction
  • scFv-CD28-CD3ζ
  • CAR Signaling Cassetes
  • CD28
    CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide costimulatory signals required for T cell activation and survival. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins which are expressed on antigen-presenting cells (APC). CD28 modulates the primary TCR/CD3ζ signal in a different fashion than the late costimulatory elements OX40 and CD28. CD28 enhances the expression of downstream regulators that impact on T-cell proliferation, death, differentiation, and effector functions. CAR+ T cells containing the CD28 endodomain showed strikingly enhanced sustained T cell activation, growth, survival. And CD28 results in a brightly expressed, stable receptor as the transmembrane domain. Including CD28 costimulatory domains in CARs led to enhanced anti-malignancy efficacy.
    CD3ζ
    CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.
  • CAR Vector Name
  • pCDCAR1
  • CAR Vector Length
  • ~8kb
  • CAR Vector Type
  • Lentiviral vector
  • CAR Generation
  • Second
  • Targeting Diseases
  • Ovarian cancer

CAR-T Cell

  • CAR-T Cell Preparation
  • Freshly human T cells were isolated from hu-mouse spleens by MACS using anti-human CD3 micro-beads, and then transduced with anti-L1CAM CAR-expressing lentiviral particles.
  • CAR-T Cell Expansion
  • The CAR virus transduced T cells were then expanded for about 326 weeks using our in-house protocol. The expanded CAR-T cells should be used immediately in hu-mice.
  • CAR-T Cell Characterization
  • The expression of human L1CAM is characterized by flow cytometry.

Quality Control

  • Cell Purity
  • >95%
  • Cell Viability
  • >90%
  • Mycoplasma Testing
  • The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
  • Sterility Testing
  • Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Shipping and Handling

  • Shipping
  • Dry Ice
  • Storage
  • Lyophilized cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
  • Handling Notes
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
    Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
    Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
  • Warnings
  • Avoid multiple freeze/thaw cycles
  • Research Use Only
  • For research use only, not for diagnostic or therapeutic use.

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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