Anti-Mesothelin (X12X46) h(scFv-CD28TM) Negative Control CAR-Jurkat (XS-1222-LX854)

Online Inquiry Datasheet

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The Anti-Mesothelin (X12X46) h(scFv-CD28TM) Negative Control CAR-Jurkat-NFAT-Luc cell line is a stable cell line derived from anti-Mesothelin CAR lentivirus transduction using Jurkat-NFAT-luciferase cell as effector cell. This transduced CAR lentiviral vector was constructed to express scFv of an anti-Mesothelin antibody linked to the CD28 transmembrane motif without any intracellular signaling domains. The CAR-Jurkat cell line constitutively expressing the firefly luciferase gene under the control of NFAT response elements to facilitate subsequent research. And the recombinant cell product targeted Mesothelin CAR-Jurkat-NFAT-Luc.

Specifications

Species
Human

Host Cell Type
T lymphocyte

Overexpressed Gene
NFAT-Luciferase Reporter

Size
Containing ≥ 2 X 10*6 / vial Frozen cells

Culture Condition
Suspension

Growth Pattern
Exponential growth phase

Formulation
Frozen cells

Cell Purity
>90%

Cell Viability
>80%

Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Shipping
Dry ice

Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C).

Warnings
Avoid multiple freeze/thaw cycles

Research Use Only
Our recombinant Jurkat cells are for research use only, not for diagnostic or therapeutic use.

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.

Tumorgenicity
Positive

Oncogenicity
Positive (In vitro life-time studies)

Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

PROTOCOL
1. Thaw CAR-Jurkat cell samples quickly in a 37°C water bath until all visible ice has melted. Thaw time for a 1 ml sample in a cryovial is 2-3 minutes. Cryovials should be cool to the touch when removed from the water bath.
2. Dilute cell/medium mixture immediately with CAR-Jurkat cell culture medium. This can be performed in a single step. The dilution medium should be between 20-37°C. A dilution ratio of 1:10 (sample:medium) or greater is recommended.
3. Plate cells appropriately according to the experimental conditions of assays.
4. Culture the control lentivirus CAR-Jurkat cells or use immediately.
Note: Jurkat cells that are used immediately after thawing have the highest level of viability.

Application
1. Compound screening;
2. Antibody screening;
3. Co-stimulatory and activation domain comparison;
4. Checkpoint inhibitors screening;
5. Safety switches and regulators of CAR-Jurkat functions study;
6. Pre-clinical study in vivo models;
7. CAR-Jurkat signaling, tumor microenvironment;
8. Proof of concept studies for clinical trials;

CAR Design

Target
Mesothelin

Target Species
Human

scFv Clone
X12X46

scFv Host Spcies
Humanized

CAR Construction
scFv-CD28TM

CAR Signaling Cassetes
Negative control CAR consists of scFv linked to the CD28 transmembrane motif without any intracellular signaling domains

CAR Vector Name
pCDCAR1

CAR Vector length
~8kb

CAR Vector Type
Lentiviral vector

CAR Generation
First

Targeting Diseases
Neuroblastoma

GeneID
10232

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.