Anti-P-1A (FAAQKAKANKAVD) TCR, Jurkat Cell Line (TCRJ-YC0807)

Online Inquiry Datasheet

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The anti-P-1A (FAAQKAKANKAVD) TCR Jurkat cell line is a stable cell line made from the anti-P-1A TCR lentivirus. The recombinant Jurkat T cell was designed to predict the MOA of P-1A-TCR, measure the P-1A-TCR specificity and screen target cells expressing P-1A. And the product can be used to treat cytomegalovirus infection.

Specifications

Cell Background
The Jurkat cell line was established from the peripheral blood of human T lymphocyte cells. Jurkat cells are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors. Jurkat cells can produce interleukin 2, and are used in research involving the susceptibility of cancers to drugs and radiation.

Cell Type
T lymphocyte

Formulation
Containing ≥ 1 X 10*6 / vial frozen cells

Cell Purity
>95%

Cell Viability
>90%

Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Applications
• Screen for activators or inhibitors of CMV signaling in a cellular context
• Characterize the biological activity of CMV and its interactions with ligands
• Predict the MOA of the TCR design
• Screen and validate CMV-expressing target cells

Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.

Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Warnings
Avoid multiple freeze/thaw cycles

Research Use Only
Our recombinant Jurkat cell are for research use only, not for diagnostic or therapeutic use.

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.

Tumorgenicity
Positive (In vitro/vivo transformation assay)

Oncogenicity
Positive (In vitro soft agarose assay and life-time studies )

Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

TCR Design

Target
P-1A

Introduction
Upon infection, CD4 T cells scan antigen-presenting cells (APCs) for pathogen-derived peptides displayed on host MHC class II proteins (pMHCII). If a T-cell–APC encounter results in intracellular signals that exceed a threshold, naïve CD4 T cells are triggered to undergo clonal expansion and acquire effector cell functions that help eliminate pathogens. Thymic selection equips mature CD4 T cells with T-cell receptors (TCRs) that preferentially react with specific foreign antigens bound to MHCII molecules. Despite the focusing of CD4 T-cell reactivity toward unique ligands, mature CD4 T cells are polyspecific, capable of being activated by multiple, distinct pMHCII ligands. T-cell polyreactivity can have both beneficial and detrimental effects during antiviral responses and may allow pathogen-derived peptides to activate autoimmune disease-inducing T cells. Thus, understanding how T-cell reactivity patterns develop has clear implications for vaccine strategies, as well as for understanding the origins of some immune and autoimmune-mediated diseases.

HLA
H2-IA^b

Common Name
P-1A

Target Species
Aequorea victoria

TCR Clone
506beta

TCR-Host Animal
Mouse

Vector Name
pCDTCR1

Vector length
~ 8 kb

Vector Type
Lentiviral vector

Targeting Diseases
Vaccinia virus infection

Epitope
FAAQKAKANKAVD

Format
Non-Modified TCR

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Anti-P-1A (FAAQKAKANKAVD) TCR, Jurkat Cell Line (TCRJ-YC0807)

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Specifications

TCR Design

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