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Anti-PSA (1L123H-f)-Megf10 CAR-MA (CARMA-W2266)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The vector of anti-PSA chimeric antigen receptor (CAR) is constructed for the engineering of macrophage cells to target human PSA and induce target cell phagocytosis. The macrophage cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-PSA antibody (1L123H-f) linked to Megf10 signaling domains. The vector product was designed for the treatment of Prostate cancer.

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Details

  • Target
  • PSA
  • Cell Type
  • Macrophage
  • Targeting Diseases
  • Prostate cancer
  • Vector Name
  • pCDCAR1
  • Vector length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Size
  • 10 µg
  • Storage
  • Store at -20°C for long term.

CAR Components

  • Promoter
  • EF1a
  • scFv
  • 1L123H-f
  • Hinge
  • CD8
  • TM
  • CD8
  • ICD
  • Megf10
  • Marker
  • GFP or N/A
  • Receptor Construction
  • scFv-hinge(CD8)-TM(CD8)-ICD(Megf10)
  • Discription of Signaling Cassetes
  • Megf10:
    Megf10 is used as the CAR intracellular signaling domain. As a phagocytosis receptor, Megf10 has cytosolic Immunoreceptor Tyrosine-based Activation Motifs (ITAMs) that are phosphorylated by Src family kinases. Based on this mechanism, Megf10 signaling can induce macrophage phagocytosis which has been demonstrated in the published article.
    It is a membrane receptor involved in phagocytosis by macrophages and astrocytes of apoptotic cells. It's a receptor for C1q, an eat-me signal, that binds phosphatidylserine expressed on the surface of apoptotic cells

Target

  • Target
  • PSA
  • Target Species
  • Human
  • Clone
  • 1L123H-f
  • Host
  • Mouse
  • Gene Name
  • Kallikrein related peptidase 3
  • Introduction
  • Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. The gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. It encodes a single-chain glycoprotein, a protease which is synthesized in the epithelial cells of the prostate gland, and is present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. The serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms.
  • Alternative Names
  • KLK3; kallikrein related peptidase 3; APS; PSA; hK3; KLK2A1

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Customer Reviews and Q&As

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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