All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.
The vector of anti-CD20 chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target human CD20. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-CD20 antibody linked to CD3ζ signaling domains. And the vector product was designed for the treatment of Recurrent/refractory CD20+ lymphoma.
CAR Construction : Fig.1 anti-CD20 binding activity of CD20-binding polypeptide compositions and immunocytokines (ICs). |
CAR Construction : Fig.2 antibody-dependent cell-mediated and complement-dependent cytotoxicity. Panel A: Antibodies tested were 2B8 (filled circles), chLeu16 (filled diamonds), and Leu16VhY/VkZ (anti-CD20 PC1) (open triangles). Panel B: Immunocytokines tested were chLeu16-IL2 (filled squares), and Leu16VhY/VkZ-IL2 (anti-CD20 PC2) (filled triangles), deglycosylated Leu16VhY/VkZ-IL2 (anti-CD20 PC3) (X's), and huKS-IL2 (open circles) as a non-binding control. Panel C shows CDC activity. |
CAR Construction : Fig.3 Antigen specificity is important for optimal anti-tumor activity. The role of tumor cell targeting was tested by comparing the activity of Leu16VhY/VkZ-IL2 (DI-Leu16-IL2) and another immunocytokine with binding specificity for EGFR, a molecule expressed at only low levels of Daudi lymphoma cells. Treatments included PBS only (X, on Days 7-11); rituximab (filled diamonds, 25 mg/kg on Days 7, 9 and 11); Leu16VhY/VkZ (DI-Leu16 antibody; open diamonds, 25 mg/kg on Days 7, 9 and 11); medium dose Leu16VhY/VkZ-IL2 (DI-Leu16-IL2; filled squares, 1 mg/kg on Days 7-11); reduced dose Leu16VhY/VkZ-IL2 (DI-Leu16-IL2, open circle, 1 mg/kg on Days 7 and 10); low dose Leu16VhY/VkZ-IL2 (DI-Leu16-IL2; open squares, 0.25 mg/kg on Days 7-11); and medium dose anti-EGFR-IL2 (filled triangle, 1 mg/kg on Days 7-11). |
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