CellRapeutics™ Human TNF - α ELISA Kit (KIT-ZP007)

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Product Name
CellRapeutics™ Human TNF - α ELISA Kit

Cat. No.
KIT-ZP007

Product format/Physical form
Linear: Measuring system linearity r> 0.990

Sensitivity
10.0 pg/mL

Specificity
Natural and recombinant human TNF-α

Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Form
Linear: Measuring system linearity r> 0.990

Purity or Quality Grade
Repeated CV < 8.0 % in batch; CV < 10.0 % between batches.

Sample Type (General)
Serum, plasma, tissue fluid, cell culture supernatant, and the like.

Product Size
8*12T/box

Storage Conditions
Storage at 2 ~ 8 °C

Shelf life
The unopened validity period is 12 months, and the reagent component after opening is valid for 1 month.

Procedure
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
1. Prepare all reagents, standard dilutions, and samples as directed in the product insert.
2. Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
100 µL Assay Diluent
3. Add 100 µL of Assay Diluent to each well.
100 µL Standard, Control, or Sample
4. Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
5. Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
200 µL Conjugate
6. Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
7. Aspirate and wash 4 times.
200 µL Substrate Solution
8. Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.
50 µL Stop Solution
9. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Helpful Tips
1. Before using this product, you must carefully read and understand this instruction manual for proper use.
2. The microporous strips in the product are disposable inspection supplies. Do not reuse them.
3. Do not mix components of different batch products when using the product, and do not use any expired components.
4. Each test should include a gradient dilution of the calibrator test to establish the correct standard curve.
5. The reagent must be equilibrated to room temperature before use. Make sure the solution components are mixed before use.
6. When using each reagent component, calibrator, and sample, a clean disposable tip must be used to avoid cross-contamination. If the prepared coloring solution turns blue, it should be discarded and reconstituted.
7. All reagents should be protected from exposure to high temperatures or sunlight.
8. If the reagent is abnormal during use, please stop using it and contact us.

Product Use Statement
This product is used to determine the concentration of IFN-γ in human serum, plasma or other samples in vitro. (Products are for scientific research only)

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.