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Intracellular Cytokine Staining (ICS) Analysis Service

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Cytokines are produced by various immune cells in response to several types of stimuli, which act as messengers for adaptive and innate immunity, allowing immune cells to communicate with each other over short distances. Cytokine analysis is very important for the study of the pathogenesis of a great number of inflammation and immunity-related diseases. Equipped with state-of-the-art facilities and experienced immunology experts, Creative Biolabs has expanded its service capabilities to offer intracellular cytokine staining (ICS) analysis service for global clients to best suit your program requirements.

The Basis of Intracellular Cytokine Staining (ICS)

Cell-mediated immune responses can be studied by measuring intracellular cytokines or cytokines secreted into serum or cell culture supernatants. It is widely believed that the level of cytokines secreted can be a true indicator of cell function, and analysis of specific intracellular cytokines can provide a snapshot of the specific cell type of cytokines of interest for researchers.

Intracellular Cytokine Staining (ICS) is an assay that can be used in basic research and preclinical studies. ICS can allow you to simultaneously measure the expression of surface markers on a cell like CD4 or CD8, as well as the expression of cytokines produced inside the cell like IL-2 or IL-17. This is helpful for researchers to study not only the cytokine is present in the cell sample, but also to identify the cell population phenotype producing specific cytokine.

Based on the fundamentals of intracellular cytokine staining (ICS), Creative Biolabs provides intracellular cytokines analysis service by flow cytometry for global clients. It is a comprehensive service capable of analyzing cytokines and other inflammatory mediators produced by cell populations of interest that allows cost-effective, precise, and fast generation of results.

Fig.1 The process for intracellular staining of cytokines for flow cytometry.

Creative Biolabs offers One-stop services ranging from initial design and optimization with well-recorded data and organized reports. If you have any questions, please contact us now for more information.

References

1. Conlon KC, Miljkovic MD, Waldmann TA. Cytokines in the treatment of cancer. Journal of Interferon & Cytokine Research. 2019 Jan 1;39(1): 6-21.
2. Saxena A, et al. Ultrasensitive Quantification of Cytokine Proteins in Single Lymphocytes From Human Blood Following ex-vivo Stimulation. Frontiers in immunology. 2018 Oct 23; 9: 2462.