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Sleeping Beauty (SB) transposon, a type of nonviral integrative vectors, provides an alternative to modify primary T cells. Creative biolabs has developed SB transposon CAR vector pSBCAR1 L1CAM (L1-9.3) h(28ζ), which is constructed for the engineering of T cells to target human L1CAM. The T cells are genetically modified through transduction with a nonviral vector expressing scFv of anti-L1CAM antibody linked to CD28 and CD3ζ signaling domains. And the vector product was designed for the treatment of Ovarian cancer.
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CAR Construction :
Fig.1 Analysis of tumor infiltrating immune cells by FACS using appropriate gating. Representative FACS blots of a Panc02-huL1CAM tumor that were treated with control mAb (IgG2a) or L1-9.3/2a. Doberstein, K., Harter, P. N., Haberkorn, U., Bretz, N. P., Arnold, B., Carretero, R., ... & Altevogt, P. (2015). Antibody therapy to human L1CAM in a transgenic mouse model blocks local tumor growth but induces EMT. International journal of cancer, 136(5), E326-E339. |
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CAR Construction :
Fig.2 To evaluate the internalisation rate of SWA11 and L1-9.3 mAbs in SKOV3ip cells the net antibody uptake assay was used. Intracellular fluorescence was measured at different time points after quenching the surface-bound Alexa-488- Salnikov, A. V., Bretz, N. P., Perne, C., Hazin, J., Keller, S., Fogel, M., ... & Altevogt, P. (2013). Antibody targeting of CD24 efficiently retards growth and influences cytokine milieu in experimental carcinomas. British journal of cancer, 108(7), 1449-1459. |
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CAR Construction :
Fig.3 Flow cytometric analysis revealed that L1CAM expression was significantly reduced in L-CI.5s cells. The mean of the reference group (shscr) was set as 100 %. Weinspach, D., Seubert, B., Schaten, S., Honert, K., Sebens, S., Altevogt, P., & Krüger, A. (2014). Role of L1 cell adhesion molecule (L1CAM) in the metastatic cascade: promotion of dissemination, colonization, and metastatic growth. Clinical & experimental metastasis, 31, 87-100. |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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