CellRapeutics™ RNA Spin Kit (CART-012CL)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

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Product Name
CellRapeutics™ RNA Spin Kit

Cat. No.
CART-012CL

General Description
96-well format using suitable vacuum manifolds or suitable centrifuges.

or fast and efficient purification in a 96-well format
6 samples can be parallel-processed with high reproducibility in under 70 min
Integrated wash plate eliminates risk of contamination
Flexible processing by vacuum or centrifugation
DNase I treatment removes genomic DNA, leading to high-quality total RNA product CellRapeuticsTM RNA Spin Kit can be used to reproducibly isolate total RNA from cultured cells, tissue, bacteria or yeast. It allows for the simultaneous processing of 96 samples via either centrifugation or vacuum-based protocols that can easily fit onto existing automated systems. The isolated RNA is of a high enough quality and quantity (up to 100 µg) for multiple downstream experiments, including sensitive enzymatic applications like quantitative reverse transcription polymerase chain reaction (QRT-PCR), primer extension, and RNase protection assays. Therefore, the RNAspin Kit is a flexible, high-throughput solution for total RNA isolation.

Applications
Used to reproducibly (well-to-well, plate-to-plate) isolate total RNA from cultured cells, tissue, bacteria or yeast
Can also be used in a fully automated flow with vacuum on common laboratory workstations
Suitable for direct use in downstream applications like Quantitative Reverse Transcriptase- PCR (QRT-PCR), Primer Extension, RNase Protection Assays, Cdna Synthesis and Microarray Analysis

Preparation Note
Total Time of Preparation: 70 min/4 preps

Binding Capacity
100 µg

Sample Size [Animal Tissue (Vacuum)]
10 mg tissue-30 mg tissue

Sample Size [Animal Tissue (Centrifuge)]
30 mg tissue

Sample Size [Cell Culture Vacuum]
2 × 106 cells

Sample Size [Cell Culture Centrifuge]
1 × 107 cells

Typical Yield [Animal Tissue Vacuum]
Up to 40 µg

Typical Yield [Animal Tissue Centrifuge]
Up to 100 µg

Typical Yield [Cell Culture Vacuum]
Up to 20 µg

Typical Yield [Cell Culture Centrifuge]
Up to 100 µg

RNA Purity [A260/A280]
1.8-2.2

Product Size
Elution Volume Min.: 50 µl Elution Volume Max.: 130 µl

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

CellRapeutics™ RNA Spin Kit (CART-012CL)

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