CBARP Membrane Protein Introduction

Introduction of CBARP

Voltage-gated calcium channels (VGCCs) are composed of an ion pore-forming Cavα1 subunit associated with several auxiliary subunits (Cavβ, Cavα2δ). CBARP, also known as voltage-dependent calcium channel beta subunit-associated regulatory protein, is a regulatory protein that is tentatively inferred to function in VGCCs. CBARP is encoded by an open reading frame of C19orf26 gene, which, based on its chromosomal location, has also been referred to as Dos. CBARP is a type I membrane protein, and the N-terminal is located outside the cell. High CBARP mRNA levels are found in the brain, pancreatic islets, and neuroendocrine cell lines, with undetectable or weak expression in other tissues.

Basic Information of CBARP
Protein Name	Voltage-dependent calcium channel beta subunit-associated regulatory protein
Gene Name	CBARP, BARP, c19orf26
Aliases	/
Organism	Homo sapiens (Human)
UniProt ID	Q8N350
Transmembrane Times	1
Length (aa)	705
Sequence	MQPTATMATAATTTTTTTATVALTTSWDNATGRPTAEPDPILDNYVLLVVVMSLFVGGTLVVLSGVLLLCKRCWDVHQRLNRAMEEAEKTTTTYLDNGTHPAQDPDFRGEDPECQDAETERFLSTSSTGRRVSFNEAALFEQSRKTQDKGRRYTLTEGDFHHLKNARLTHLHLPPLKIVTIHECDSGEASSATTPHPATSPKATLAIFQPPGKALTGRSVGPSSALPGDPYNSAAGATDFAEISPSASSDSGEGTSLDAGTRSTKAGGPGAAAGPGEAGPGSGAGTVLQFLTRLRRHASLDGASPYFKVKKWKLEPSQRAASLDTRGSPKRHHFQRQRAASESTEQEEGDAPQEDFIQYIARAGDAVAFPHPRPFLASPPPALGRLEAAEAAGGASPDSPPERGAGSAGPEQQQPPLEPDAERDAGPEQAQTSYRDLWSLRASLELHAAASDHSSSGNDRDSVRSGDSSGSGSGGAAPAFPPPSPPAPRPKDGEARRLLQMDSGYASIEGRGAGDDTEPPAAPARPRSPRAWPRRPRRDYSIDEKTDALFHEFLRHDPHFDDTPAAARHRARAHPHARKQWQRGRQHSDPGARAAPALAGTPAPPAGAARPARAPLRRGDSVDGPPDGRTLGGAGDDPAIPVIEEEPGGGGCPGSGLCVLPSGSVLDKLAAGLDERLFPPRLAEPVVATPALVAAAPTSPDHSPA

Function of CBARP Membrane Protein

CBARP interacts with all Cavβ subunit isoforms via two cytoplasmic domains, affecting their subcellular localization and inhibiting VGCC activity. CBARP interacts at the α1 interaction domain-binding pocket in Cavβ and interferes with the binding between Cavβ and Cavα1. CBARP does not affect cell surface expression of Cavα1, but inhibits Ca²⁺ channel activity at the plasma membrane, resulting in inhibition of Ca²⁺-induced exocytosis. Therefore, CBARP can regulate the localization of Cavβ and its binding to the Cavα1 subunit to negatively regulating VGCC activity.

Fig.1 Subunit composition of voltage-gated Ca²⁺ channels. CBARP is located between α subunit and β subunit. (Buraei, 2010)

Application of CBARP Membrane Protein in Literature

Nakao A., et al. Comprehensive behavioral analysis of voltage-gated calcium channel beta-anchoring and -regulatory protein knockout mice. Front Behav Neurosci. 2015, 9: 141. PubMed ID: 26136667

This article uses mouse experiments to demonstrate that CBARP is involved in the regulation of locomotor activity and emotionality. Moreover, CBARP knockout mice can be used as a unique tool to study the pathogenesis/pathophysiology of schizophrenia and related disorders.
Béguin P., et al. BARP suppresses voltage-gated calcium channel activity and Ca²⁺-evoked exocytosis. J Cell Biol. 2014, 205(2): 233-49. PubMed ID: 24751537

This article demonstrates that CBARP regulates the localization of Cavβ and its association with the Cavα1 subunit, thereby negatively regulating VGCC activity.
McCabe M.T., et al. Homozygous deletion of the STK11/LKB1 locus and the generation of novel fusion transcripts in cervical cancer cells. Cancer Genet Cytogenet. 2010, 197(2): 130-41. PubMed ID: 20193846

This article determines that the expression of CBARP protein may be associated with breast cancer by exploring the genotype of breast cancer cell lines.

CBARP Preparation Options

Membrane protein studies have advanced significantly over the past few years. Based on our versatile Magic™ membrane protein production platform, we could offer a series of membrane protein preparation services for worldwide customers in reconstitution forms or membrane protein analysis. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-CBARP antibody development services.

During the past years, Creative Biolabs has successfully generated many functional membrane proteins for our global customers. We are happy to accelerate the development of our clients’ programs with our one-stop, custom-oriented service. For more detailed information, please feel free to contact us.

Reference

Buraei Z and Yang J. (2010). The ß subunit of voltage-gated Ca²⁺ channels. Physiol Rev. 90: 1461-1506.

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