CDH17 Membrane Protein Introduction

Introduction of CDH17

CDH17, also known as intestinal peptide-associated transporter HPT-1 or liver-intestine cadherin, consists of seven homologous repeated domains without the His-Ala-Val motif of the N-terminal domain and has only 20 amino acid residues for its CDH17 cytoplasmic portion, a short COOH-terminal for possible cell adhesion function. This protein belongs to the cadherin superfamily which is responsible for intercellular conjunction. The function of CDH17 is mainly in cell-cell adhesion and it may be implicated in tumorigenesis.

Function of CDH17 Membrane Protein

High expression of CDH17 is associated with gastric cancer, colorectal cancer, liver cancer, pancreatic cancer, and cholangiocarcinoma, suggesting that CDH17 may play an important role in tumors. CDH17 has the ability to modulate Ca²⁺-dependent homophilic cell-cell adhesion without relying on cytoskeletal interactions, so it may play an important role in tumor metastasis. CDH17 knockdown may result in inactivation of Wnt signaling, thereby inhibiting the activity of cancer cell invasion. More importantly, the loss of CDH17 may lead to increased expression of placental growth factor and metal-reactive transcription factor-1, which is thought to increase tumor invasiveness and thereby regulate angiogenesis in human cancers. In addition, by activating the NFκB signaling pathway, CDH17 can also induce lymph node (LN) metastasis.

Fig.1 Schematic diagram of the regulatory and signaling network of CDH17 in liver cancer cells. At the transcriptional level, expression of CDH17 is at least regulated by transcription factors such as CDX2 and HNF1α. Regarding the downstream cellular pathway, it was shown that CDH17 activates the Wnt signaling pathway. (Lee NP, 2010)

Application of CDH17 Membrane Protein in Literature

1. Choi B., et al. Plasma expression of the intestinal metaplasia markers CDH17 and TFF3 in patients with gastric cancer. Cancer Biomark. 2017, 19(3):231-39. PubMed ID: 28453457
   
   

   This article compared the serum levels of CDH17 in patients with stage II and III gastric cancer and healthy controls. Compared with the control group, the expression of CDH17 was significantly increased in patients with stage II and III gastric cancer (p= 0.023 and p= 0.037, respectively).

2. Snow A.N., et al. Expression of cadherin 17 in well-differentiated neuroendocrine tumours. Histopathology. 2015, 66(7):1010-21. PubMed ID: 25388236
   
   

   CDH17 is a sensitive marker for midgut well-differentiated neuroendocrine tumors (WDNETs). Immunohistochemical analysis of different anatomical sites showed that the CDH17+/CDX2-/TTF1-phenotype was sensitive (92%) and specific (91%) for WDNETs.

3. Li R., et al. Inhibition of CDH17 gene expression via RNA interference reduces proliferation and apoptosis of human MKN28 gastric cancer cells. Int J Oncol. 2017, 50(1):15-22. PubMed ID: 27909714
   
   

   Using a lentiviral system as a delivery mediator of RNA interference, it was found that inhibition of CDH17 can result in reduced proliferation and an increase in apoptosis of the gastric cancer cell line MKN28 in vitro and a significant reduction in tumorigenicity in vivo.

4. Altree-Tacha D., et al. CDH17 is a more sensitive marker for gastric adenocarcinoma than CK20 and CDX2. Arch Pathol Lab Med. 2017, 141(1):144-50. PubMed ID: 28029907
   
   

   In this article, immunohistochemistry showed that CDH17 positive rate was higher in colon cancer, gastric adenocarcinoma and esophageal adenocarcinoma than CK20 and CDX2. It indicated that CDH17 was a specific and more sensitive marker in the gastrointestinal tract.

5. Andersen C.L., et al. Whole-exome sequencing and genome-wide methylation analyses identify novel disease associated mutations and methylation patterns in idiopathic hypereosinophilic syndrome. Oncotarget. 2015, 6(38):40588-97. PubMed ID: 26497854
   
   

   The article analyzed purified eosinophils from patients with the idiopathic hypereosinophilic syndrome (IHES) by next-generation whole-exome sequencing and compared DNA methylation profiles from reactive eosinophilic conditions to known clonal and suspected clonal eosinophilia. It showed mutations in CDH17 gene was associated with IHES.

CDH17 Preparation Options

Membrane protein studies have advanced significantly over the past few years. Based on our versatile Magic™ membrane protein production platform, we could offer a series of membrane protein preparation services for worldwide customers in reconstitution forms or membrane protein analysis. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-CDH17 antibody development services.

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During the past years, Creative Biolabs has successfully generated many functional membrane proteins for our global customers. We are happy to accelerate the development of our clients’ programs with our one-stop, custom-oriented service. For more detailed information, please feel free to contact us.

Reference

1. Lee N P, et al. (2010). Role of cadherin-17 in oncogenesis and potential therapeutic implications in hepatocellular carcinoma. Biochim Biophys Acta. 1806(2), 138-45.

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