cDNA Library Introduction

The applications of cDNA libraries are wide, including gene isolation, cloning, expression, discovery, and functional studies. With the help of phage display technology, the whole cDNA product can be expressed on the phage surface by fusion proteins, which allows the identification of a large number of candidate proteins and the isolation of specific binders to various targets. However, traditional phage display technology still faces some challenges in constructing complex cDNA libraries, which can lead to lower yields of functional clones. Based on our rich practical experience and advanced working platform, Creative Biolabs provides comprehensive services to support phage display library construction. Our services include but are not limited to scaffold libraries, peptide libraries, and immune antibody libraries.

cDNA. (Creative Biolabs Authorized)

cDNA Library

cDNA library construction is to reverse transcribe mRNA into cDNA in vitro, link it with appropriate plasmid vectors, transform recipient bacteria, and propagate and amplify. Finally, a cDNA clone population containing the entire mRNA information of the tissue or cell was obtained. It has unique advantages for studying the expression status of genomes in specific cells and identifying the functions of expressed genes. This advantage makes it more widely used in the study of life phenomena such as ontogeny, cell differentiation, cell cycle regulation, cell senescence, and cell death regulation. Currently, cDNA libraries have a wide range of applications. cDNA libraries can be used to screen specific genes and also study gene expression. In addition, in the biomedical field, cDNA libraries can be used for screening drug targets and also for studying the structure and function of genes. And phage display cDNA library technology combines the surface display of target proteins with affinity screening technology to realize the unification of genotype and phenotype. In the field of phage display library technology, phage display cDNA library technology is widely used in the study of various natural ligand-receptor interactions. And this is the basis for studying the structure and function of various pathogenic microorganisms. Therefore, phage display cDNA library technology plays an important role in the diagnosis of pathogenic microorganisms, the study of pathogenic mechanisms, and the development of vaccines and drugs.

The classical method of cDNA library construction is to use Oligo(dT) as a reverse transcription primer or random primer, add an appropriate linker to the synthesized cDNA, and connect it to the appropriate vector to obtain the library. Usually, the cDNA library is constructed by using specific tissue or cell mRNA as a template, reverse transcription of complementary DNA (cDNA), and appropriate vector (commonly a phage or plasmid vector), and then transformed into recipient bacteria to form recombinant DNA clone groups. The collection of cDNA clones containing all the mRNA information of a cell is called the cDNA library of that tissue or cell. The cDNA library is tissue- or cell-specific because it reflects the genes encoding proteins that are expressed in a particular tissue or cell at a particular developmental stage.

Creative Biolabs has a wealth of knowledge and experience in phage display library construction. We would be happy to discuss with you our knowledge and experience in cDNA library construction.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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