As a long-term undisputed global pioneer in the field of phage display, Creative Biolabs has developed a comprehensive service portfolio based on our state-of-the-art technical platforms. Currently, Creative Biolabs is pleased to offer this exclusive service of T4 phage display library construction to our customers around the world.
First established in the 1980s, phage display has been a pivotal laboratory technique widely utilized in a variety of research fields, including antibody engineering, drug discovery, vaccine design, epitope mapping, and so on. By displaying certain molecules on the surface of genetically modified phages, a direct connection between genotype and phenotype can be formed, thus enabling the high-throughput analysis of vast amount of variants. Comparing with the most widely used filamentous phage system (i.e. M13), T4 phage display system also provides a promising alternative for its special merits.
Bacteriophage T4 is distinct from M13 in many aspects. T4 is of much larger size, tailed structure and double-stranded DNA (dsDNA) genome encoding 50 different proteins. Based on the appearance, T4 phage is divided into three parts: a head, a fistulous and contractile neck for DNA injection and a tail part for recognizing and binding to the bacteria surface. The viral head, where T4 preserves its DNA, has an icosahedral structure mainly composed of three coat proteins: gp23, gp24 and gp20. In addition, there exist two non-essential proteins, HOC and SOC, decorated on the icosahedral head together with other coat proteins. In addition, T4 undergoes an exclusively lytic lifecycle, which is different with M13, meaning that after infection progeny phage particles are assembled in the cytoplasm and released by lysis of the host bacterium.
Fig 1. Structure of the bacteriophage T4 head.1
T4 phage display system employs two dispensable capsid proteins, HOC and SOC, to genetically fuse with exogenous sequences thus displaying the specific molecules on the head surface. Both N- and C-termini are accessible to foreign insertions without impacting the infectivity and viability of T4. Furthermore, T4 phages have a lytic lifecycle without going through membrane extraction process, which might limit the insertion size, affect the protein confirmation as well as cause toxicity to the host. Hence, T4 display system can provide an optimized solution to overcome the main barriers of M13 phage display.
Three available approaches for T4 library construction:
Generally, T4 phage display system can offer major advantages over other display systems including:
T4 phage display has revealed great potential in various research and pharmaceutical fields, especially multicomponent vaccine development. It provides a promising alternative when conventional M13 phage display does not work well. With our extensive experience and unparalleled technologies after years of accumulation, Creative Biolabs has confidence in bringing professional services based on T4 phage display systems, assisting our clients to pave the way to revolutionary scientific progress.
A T4 phage display library is used to display peptides or proteins on the surface of the T4 bacteriophage. This is achieved by inserting DNA sequences encoding peptides or proteins into the phage's genome, specifically into the genes that encode the phage's capsid proteins. As the phage replicates, it displays the inserted peptides on its surface. Researchers can then screen these displayed peptides for their ability to bind to specific targets, making the T4 phage display library useful in various applications, including drug discovery and diagnostic development.
The T4 phage display library is employed in numerous research fields, such as drug discovery, vaccine development, and the study of protein-protein interactions. Its ability to display peptides or proteins in a high-density format on the phage surface makes it particularly valuable for identifying ligands that bind to specific targets with high affinity. Additionally, the T4 phage display system is used in the development of diagnostic assays and in the exploration of novel therapeutic candidates.
Construction of a T4 phage display library involves the insertion of DNA sequences encoding the desired peptides or proteins into the genome of the T4 bacteriophage. This is typically done by incorporating these sequences into genes encoding the phage's capsid proteins, allowing the peptides to be displayed on the surface of the phage particle. The library is then propagated in a bacterial host, resulting in a diverse collection of phages, each displaying a different peptide. These phages can be screened against specific targets to identify strong binders.
The T4 phage display library offers several advantages, including a larger capsid that can accommodate larger peptides or even whole proteins, providing greater flexibility in the types of molecules that can be displayed. Additionally, the T4 phage has a robust life cycle and can be produced in large quantities, making it suitable for high-throughput screening applications. The T4 system is also highly stable and can display peptides or proteins in a native-like conformation, which is critical for maintaining their biological activity during screening.
The T4 phage display library allows researchers to screen a vast number of peptides or proteins against a specific target, such as a disease-related protein. By selecting peptides that bind strongly to the target, researchers can identify potential therapeutic candidates that may inhibit or modulate the target's function. This approach is particularly valuable in drug discovery, where identifying novel binding partners can lead to the development of new drugs or therapies.
The T4 phage system is capable of displaying peptides in a multivalent format, meaning that multiple copies of the same peptide can be displayed on a single phage particle. This multivalent display enhances the binding strength (avidity) of the peptide to its target, which is particularly useful when selecting peptides that require strong interactions for effective binding. The multivalent nature of the T4 phage display system is a significant advantage in applications such as vaccine development and therapeutic screening.
Use the resources in our library to help you understand your options and make critical decisions for your study.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
USA:
Europe: Germany: |
|
Call us at: USA: UK: Germany: |
|
Fax:
|
|
Email: info@creative-biolabs.com |