Many different novel strategies have been developed to derive genetically modified tumor cells and use them as cellular vaccines to induce useful antitumor immunity. Genetically modified tumor cells with IL-15 represent one of the novel promising approaches. With our cell line development services, scientists at Creative Biolabs are proficient at engineering tumor cell lines with IL-15 overexpression. We are confident to handle all aspects of your engineering tumor cell workflow, from start to finish.

IL-15 Functions in T Cell Activation

Interleukin (IL)-15 is a cytokine that promotes the development and homeostasis of a group of lymphocytes. IL-15 shares many functions in regulating both adaptive (stimulation of T cell proliferation and induction of cytotoxic T lymphocytes) and innate (activating natural killer (NK) cells) immune responses. IL-15 shares biological activities with IL-2. Additionally, unlike IL-2, IL-15 has several added benefits, including the lack of stimulation on regulatory T cell populations, reduced activation-induced cell death (AICD), and lower toxicity.

• IL-15 induces the activation, proliferation and survival of T cells.
• IL-15 induces the maintenance of high-avidity, antigen-specific CD8+memory T cells in the long term.
• IL-15 can also affect T-cell numbers by influencing T-cell trafficking.
• IL-15 regulates both proliferation and antibody production by activated B cells.
• IL-15 induces the development, persistence and activation of NK and NKT as well as γ/δT cells.
• IL-15 activates the dendritic cells and macrophages to produce pro-inflammatory cytokines that participate in T cell activation and differentiation.

Fig.1 Schematic of the biological properties of IL-15. (Patidar, et al., 2016)

Studies Performed with IL-15 Overexpressing Tumor Cells

• A study showed a whole-cell CT26 colon cancer vaccine, which secretes a complex of IL-15 and IL-15Rα, could trigger anti-tumor response via CD4 T, CD8 T, and NK cell-dependent cytotoxicity.
• A study indicated the human small cell lung cancer cell line, N592, genetically engineered to secrete IL-15, showed a reduced tumor growth rate.

Engineering Cancer Cells with IL-15 Overexpression Service

Designing specific active immunotherapy protocols utilizing cytokine gene-modified tumor cells extends the realm of cancer therapeutic possibilities. To promote the future of cancer immunotherapy, Creative Biolabs provides cell line engineering services to integrate the IL-15 gene into the host cell of your interest. Guaranteed by professionals with comprehensive experience, we offer a full package covering from vector design and cell line generation, to assay development, all serving to fulfill your needs. We have access to a range of gene transfer technologies and an expert team experienced at working with a variety of cancer cell lines, including adherent and suspension cells. We can provide small to large-scale custom cell lines with a fast turnaround and complete validation.

We help choose the most effective transfection method for your application and cell type, including:

Our cancer cell engineering services cover a range of different cell lines, including:

Creative Biolabs provides you with state-of-the-art cell line engineering and production services. Each project is customized for the desired deliverables by working directly with our team of highly trained scientists using a milestone-measured process. Please contact us for consultations on study design, choice of vector, and how we can enable your imagination!

Reference

1. Patidar, M.; et al. Interleukin 15: A key cytokine for immunotherapy. Cytokine & growth factor reviews. 2016, 31: 49-59.

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