M6PR Membrane Protein Introduction

Introduction of M6PR

Mannose 6-phosphate receptor (M6PR) is encoded in humans by the M6PR gene. In the field of biochemistry and cell biology, it is also known as the cation-dependent mannose-6-phosphate receptor (CD-MPR). M6PR is a type I transmembrane protein with a single transmembrane domain and a C-terminus located on the cytoplasmic side of the lipid membrane. M6PR is approximately 46 kDa in size and has a relatively short cytoplasmic tail with a cytoplasmic M6P binding domain consisting of 157 amino acid residues, which is involved in dimerization.

Basic Information of M6PR
Protein Name	Cation-dependent mannose-6-phosphate receptor
Gene Name	M6PR
Aliases	CD-MPR, MPR46, SMPR, CD-mannose-6-phosphate receptor, cation dependent
Organism	Homo sapiens (Human)
UniProt ID	P20645
Transmembrane Times	1
Length (aa)	277
Sequence	MFPFYSCWRTGLLLLLLAVAVRESWQTEEKTCDLVGEKGKESEKELALVKRLKPLFNKSFESTVGQGSDTYIYIFRVCREAGNHTSGAGLVQINKSNGKETVVGRLNETHIFNGSNWIMLIYKGGDEYDNHCGKEQRRAVVMISCNRHTLADNFNPVSEERGKVQDCFYLFEMDSSLACSPEISHLSVGSILLVTFASLVAVYVVGGFLYQRLVVGAKGMEQFPHLAFWQDLGNLVADGCDFVCRSKPRNVPAAYRGVGDDQLGEESEERDDHLLPM

Function of M6PR Membrane Protein

The primary function of M6PR is the sorter and deliverer of lysosomal enzymes. The lysosomal enzymes are sorted and delivered by receptor-mediated transport by binding to the extracellular domain of M6PR. Lysosomal enzymes are transported to lysosomes via two M6PR-dependent pathways. One pathway is that the lysosomal enzyme marker M6P in the Golgi complex is combined with M6PR and then transported into the Golgi apparatus. The other pathway is the binding of M6P-containing lysosomes in the extracellular matrix to M6PR on the cell membrane to transport lysosomal enzymes. In both pathways, a receptor-ligand complex is formed by binding of a lysosomal enzyme to M6PR and then transported to a lysosome. Besides, in a low pH environment, the receptor and ligand are separated and the M6PR is recycled back to the Golgi complex or plasma membrane.

M6PR Membrane Protein Introduction Fig.1 The structure of M6PR.

Application of M6PR Membrane Protein in Literature

Lemansky P., et al. The cation-independent mannose 6-phosphate receptor is involved in lysosomal delivery of serglycin. Journal of Leukocyte Biology. 2007, 81(4):1149-1158. PubMed ID: 17210618

The authors observed that M6PR and Sortilin bound to immobilized CS and concluded that CI-MPR was involved in lysozyme and granule-targeted serum glycine and basic proteins, such as lysozymes associated with proteoglycans in hematopoietic cells.
Chen J.J., et al. Mannose 6-phosphate receptor dependence of varicella zoster virus infection in vitro and in the epidermis during varicella and zoster. Cell. 2004, 119(7):915-926. PubMed ID: 15620351

The article found that intracellular MPRci appeared to transfer the newly enveloped VZV to late endosomes, suggesting that the plasma membrane MPRci is required for cell-free VZV entry.
Yang E.B., et al. Genetic changes and expression of the mannose 6-phosphate/insulin-like growth factor II receptor gene in human hepatitis B virus-associated hepatocellular carcinoma. International Journal of Molecular Medicine. 2003, 11(6):773-778. PubMed ID: 12736721

The study showed that the expression of M6P / IGF2R mRNA in HCC tissues of HBV-positive patients was lower than that of non-tumor tissues, suggesting that M6P / IGF2R may participate in the level of HBV-related hepatocarcinogenesis through the regulation of its expression.
Dahms N.M., et al. 46 kd mannose 6-phosphate receptor: cloning, expression, and homology to the 215 kd mannose 6-phosphate receptor. Cell. 1987, 50(2):181-92. PubMed ID: 2954652

The authors isolated a cDNA clone encoding the entire sequence of the bovine 46kd cation-dependent mannose 6-phosphate (CD Man-6-P) receptor and found that it may contain 279 amino acid sequences, a peptide containing a signal peptide sequence and a transmembrane domain.
Pohlmann R., et al. Cloning of a cDNA encoding the human cation-dependent mannose 6-phosphate-specific receptor. PNAS. 1987, 84(16):5575-5579. PubMed ID: 2441386

The authors isolated a complementary DNA clone of human cation-dependent mannose 6-phosphate-specific receptor from a human placental library of λgt11 and performed related biological information analysis.

M6PR Preparation Options

To obtain the soluble and functional target protein, the versatile Magic™ membrane protein production platform in Creative Biolabs enables many flexible options, from which you can always find a better match for your particular project. Besides, we also provide customized anti-M6PR antibody development services based on our versatile Magic™ anti-membrane protein antibody discovery platform.

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