Close
Close

Magic™ Antibody Discovery - Human Sonic Hedgehog / Shh (24-197) Membrane Protein, Partial, -His tag (CAT#: MP0408F)

Datasheet
This membrane protein is Human Sonic Hedgehog / Shh (24-197). It has been tested in SDS-PAGE. We provide this protein to facilitate your membrane protein antibody discovery and development.

Product Specifications

  • Host Species
  • Human
  • Target Protein
  • Sonic Hedgehog / Shh
  • Protein Length
  • ECD
  • Molecular Weight
  • The protein has a calculated MW of 20.4 kDa. The protein migrates as 23-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
  • Sequence
  • AA Cys 24 - Gly 197 (Accession # NP_000184).

Product Description

  • Application
  • SDS-PAGE
  • Expression Systems
  • HEK293
  • Tag
  • His tag at the C-terminus
  • Protein Format
  • Soluble
  • Reconstitution
  • Please see Certificate of Analysis for specific instructions.
  • Endotoxin
  • <1.0 EU/μg by the LAL method
  • Purity
  • >95% as determined by SDS-PAGE.
  • Buffer
  • Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Target

  • Target Protein
  • Sonic Hedgehog / Shh
  • Full Name
  • sonic hedgehog signaling molecule
  • Introduction
  • This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.
  • Alternative Names
  • TPT; HHG1; HLP3; HPE3; SMMCI; ShhNC; TPTPS; MCOPCB5; sonic hedgehog protein; shh unprocessed N-terminal signaling and C-terminal autoprocessing domains;sonic hedgehog homolog

Customer reviews and Q&As    

Related Products
Online Inquiry
CONTACT US
USA:
Europe:
Germany:
Call us at:
USA:
UK:
Germany:
Fax:
Email:
Our customer service representatives are available 24 hours a day, 7 days a week. Contact Us
© 2024 Creative Biolabs. | Contact Us