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Magic™ Simplified Cell-free Membrane Protein Expression in E. coli Lysate (CAT#: MPCFK-1-1)

Datasheet
Magic™ Simplified Cell-free Protein Expression in E.coli Lysate kit is designed for protein synthesis using E.coli extracts. This kit contains an optimized E.coli extract and an optimized Master mix. It contains optimized all necessary components for E.coli cell-free protein expression, such as T7 RNA polymerase, ribosomes, amino acids, rNTPs, and appreciate salts to express high levels of recombinant proteins. This kit contains reagents for expresssion of protein directly from a variety of DNA templates which contains T7 promoter, T7 terminator and RBS (ribosomal binding site). This flexible system expresses up to 300 ug/mL of protein in 3 hours.

Product Specifications

  • Lysate Type
  • E. coli Lysate
  • Size
  • 24 reactions; 5 reactions; 1 reaction
  • Reaction Volume
  • 45 µL per reaction/1 mL per reaction/10 mL per reaction
  • Expected Yield of Positive Control
  • 0.45 mg/ml
  • Components
  • E.coli Extract
    Master mix
    DEPC Buffer
    Control DNA
  • Storage Conditions
  • Store at -80°C.

Customer reviews and Q&As    

Customer Review

The protein expression is abundant

The protein expression level met the needs of subsequent experiments.
2023.1.03

Q&As

What are the formats of CF synthesis methods?

1.The batch format utilizes one vesicle comprising both reaction medium (RM) and feeding solution (FS).
2.Continuous-flow CF methodology
3.Continuous-exchange CF
4.Bilayer format
2023.01.04

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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