Phage Display Antibody Library Introduction

The classical method of antibody generation heavily relies on the hybridoma technique. However, this method involves time-consuming steps such as animal immunization and cell fusion, taking months to prepare. In contrast, phage display technology offers a substantial time-saving advantage by bypassing these initial stages. Additionally, phage display technology provides a versatile source of antibodies that can cross species barriers, yielding human antibodies directly. It can also be applied to various scenarios, including antibody affinity maturation. Drawing from our extensive experience, Creative Biolabs provides comprehensive services in phage display antibody libraries and premade antibody libraries to support the development of phage display technology.

M13 Phage. (Creative Biolabs Authorized)

Phage Display Antibody Library

In the late 1980s, the integration of phage display technology, PCR technology, and genetic engineering antibody techniques gave rise to phage display antibody library technology. The essence of a phage display library is to clone and fuse the entire repertoire of antibody heavy chain and light chain genes with the structural proteins, primarily P3 protein, of phage particles. This fusion occurs in the form of single-chain antibodies (scFv), single-domain antibody (VH or VL), or Fab antibody, and they are displayed on the surface of phage particles. Generally, phage antibody libraries fall into three major groups, categorized by the type of antibody they contain: naïve antibody libraries, semi-synthetic antibody libraries, and synthetic antibody libraries.

Naïve antibody libraries primarily consist of y B-lymphocytes sourced from blood, bone marrow, spleen, and tonsils of genetically derived human or animal donors. The advantages of these natural antibody libraries include the ability to obtain human antibodies, the capacity to target a wide range of natural antigens, and the potential to directly acquire high-affinity antibodies. However, they have certain disadvantages, such as limited library capacity compared to semi-synthetic and synthetic antibody libraries, and the time and labor involved in their construction.

Semi-synthetic antibody libraries are composed of antibody libraries created by randomly synthesizing and amplifying DNA sequences in the CDR3 region. These libraries are mainly based on the framework region and CDR1 and CDR2 regions in natural antibody libraries. The CDR3 region is subject to randomization, altering diversity by varying the sequence and length to increase library capacity. Some designers have achieved diversity levels as high as 10⁸ by randomizing all six CDR regions.

Synthetic antibody libraries are entirely based on information derived from the antibody gene library, involving purely artificial synthesis. Creating fully synthetic antibody libraries demands an in-depth understanding of the CDR region of the antibody. It involves preserving the common or backbone portion of the CDR region while designing interchangeable gene regions. This approach allows for a high degree of randomization, resulting in a substantial library capacity.

Libraries with large capacity and rich diversity are easier to screen for ideal antibodies. Therefore, when performing phage display library construction, capacity and diversity are critical considerations. Theoretically, fully synthesized antibody libraries have the largest capacity. However, certain factors, such as conserved sequences in the backbone region, also impact. In addition, during performing phage display library screening, it is possible to create immune antibody libraries by utilizing spleens from animals immunized with specific antigens. This approach can significantly enhance the success rate of identifying antibodies against those particular antigens.

More Details on Phage Display Antibody Library

Immune Antibody Library Introduction
Naïve Antibody Library Introduction
Semi-synthetic Antibody Library Introduction
Synthetic Antibody Library Introduction

Creative Biolabs possesses extensive expertise in the field of antibody phage display. We are eager to engage in discussions with you regarding our knowledge and hands-on experience with phage display antibody libraries.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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