Naïve Antibody Library Introduction

Natural antibody libraries play a crucial role in phage antibody display libraries. Theoretically, these libraries can be screened to obtain antibodies that specifically bind to virtually any antigen. However, these natural antibody genes lack the in vivo rearrangement and mutation processes, often resulting in a challenge to obtain high-affinity antibodies. Consequently, antibodies identified through natural antibody libraries often require further affinity maturation and modification to achieve the desired properties. With our extensive practical experience and advanced research platform, Creative Biolabs offers comprehensive services for the research and development of antibody libraries for in vitro display and antibody discovery services.

B cells and Antibodies. (Creative Biolabs Authorized)

Naïve Antibody Library

Naïve antibody libraries are constructed using lymphocytes that have not undergone immunization. These libraries typically source genes from B-lymphocytes found in the blood, bone marrow, spleen, and tonsils of both human and animal donors, with the corresponding antibodies obtained through gene amplification. Human antibody libraries can generate fully human antibody V genes directly, eliminating the need for laborious subsequent humanization processes. Furthermore, these libraries encompass all antibody genes from both within and outside the bone marrow, making them suitable for selecting antibodies targeting a wide array of antigens. The library capacity must be substantial, starting at a minimum of 10⁹, given the estimated magnitude of antigen species in nature.

The CAT1.0 library, initially developed by Cambridge Antibody Technology (now part of MedImmune/AstraZeneca), stands as the first large, all-human natural antibody library. It was created by amplifying the variable region genes of heavy and light chain genes from peripheral blood B cells of 43 healthy individuals using PCR and subsequently combining them randomly to form single-chain variable fragments (scFvs). This library boasts a size of 1.4×10¹⁰. The CAT2.0 library represents an upgraded version of the CAT1.0, with an even larger capacity of up to 1.29×10¹¹.

Large-capacity natural antibody libraries offer several advantages. They are versatile, capable of targeting all antigens, including self-antigens and non-immunogens. They allow for rapid antibody generation, particularly human antibodies. However, it's essential to acknowledge their drawbacks. Constructing natural antibody libraries is a time-consuming and labor-intensive process, rife with numerous unknown and uncontrollable factors that can affect library capacity and quality. In addition, antibodies obtained from natural antibody libraries tend to exhibit lower affinity and a reduced abundance of antibodies specific to particular antigens due to high screening background.

Creative Biolabs has a wealth of knowledge and experience in phage display library construction. We are delighted to share our expertise and practical knowledge in the field of antibody library screening and naïve antibody library construction.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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