Polyclonal sdAb Generation Service
Creative Biolabs has long-term devoted to the development and application of sdAbs. Based on our advanced platforms and extensive experience, our scientists are confident in offering the best and most suitable polyclonal sdAb generation services to meet your specific requirements.
Camelid IgG Introduction
Different from any other known antibodies, the camelid IgG antibodies consist of three subclasses. Thereinto, IgG1 is composed of conventional antibodies, while IgG2 and IgG3 lack the light chains and consist of dimers of short heavy chains characterized by a normal Fc region without a CH1 domain. Compared with the conventional antibodies, IgG2 and IgG3 antibodies (also known as heavy chain only antibody), as well as their antigen-binding domain (VHH), present great advantages due to the small size. According to the different species, IgG isotype can be further divided into:
Llama/Alpaca: IgG1a, IgG1b, IgG2b, IgG2c, IgG3.
Camel: IgG1a, IgG1b, IgG2a, IgG2c, IgG3.
Comprehensive Strategies for Camelid Polyclonal Antibody Generation
Taking the advantages of our deep understanding of camelid antibodies, Creative Biolabs can offer three elaborate stages to satisfy your various demands for camelid polyclonal antibody generation, which can let you obtain the real polyclonal single domain antibody.
Starting with a properly designed antigen and immunization strategy, favorable immune response is expected to be triggered in 70 days. The blood will be collected after the confirmation of titration ELISA for further purification processes. Our scientists can tailor three strategies according to the different demands of final deliverables.
Generation of Antigen-Specific Polyclonal Camelid Antibody
The antigen-specific affinity purification will be employed as the main process, in which the antigen of interest will be coated to the column and then we will perform the purification for a few rounds. For polyclonal antibody purification, it is essential to avoid the co-purification of non-specific immunoglobulins. This approach can contribute to obtaining almost all antibodies (full-length format with different isotypes) that can recognize the antigen. It is also a foundation for the other two strategies.
Generation of Polyclonal Heavy Chain Only Antibody
The classic Protein A and Protein G columns will be employed in this strategy. With this subsequent purification process, our scientists can properly separate the isotype of obtained antigen-specific pAbs and also eliminate some unwanted binders. This approach can meet your demands for generating heavy chain only pAbs or pAbs with specific IgG isotype (IgG1, IgG2, or IgG3).
Generation of Polyclonal Single Domain Antibody
For the demands of purified polyclonal single domain antibody (VHH only), our scientists can also achieve such purpose by integrating properly designed digestion strategy. Going through a series of digestion, washing, and elution steps, polyclonal single domain antibodies can be finally obtained from either IgG2 or IgG3.
Creative Biolabs is a leading service provider that focuses on sdAb generation and development. We have successfully accomplished a series of camelid polyclonal antibody generation projects for our customers all over the world. If you are interested in our service, please do not hesitate to contact us for more details.
Case Study
We are offering highly customized CRO services to assist your Single Domain Antibody (sdAb) related projects. Please Contact Us for more details.
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