Benefited from the high stability, sdAbs are being exploited for several applications ranging from therapeutics and diagnostics to biotechnology. With our extensive experience and deep understanding of sdAb, Creative Biolabs has set up a unique platform for sdAb stability improvement, which can assist your sdAb development with the highest efficiency and the best quality.

Stability of sdAb

Successful application as therapeutic and diagnostic tools require not only high affinity and specificity but also excellent biochemical and biophysical properties. sdAbs are gaining a reputation as superior recognition elements as they combine the advantages of specificity and affinity with high stability and solubility. Inherently, sdAbs are extraordinarily resistant under different harsh and extreme conditions and remarkably stable while maintaining full antigen-binding capacity. Altogether, most sdAbs exhibit high stability against elevated temperature, high pressure, or chemical denaturants. These peculiar biochemical and biophysical properties of sdAbs have stimulated their introduction in various applications such as in vivo immunomodulation, diagnostic detection, and protein purification. Furthermore, the stability of sdAbs under extreme conditions and their proteolytic resistance make them best suitable for use in alternative routes of administration (i.e., oral administration).

Fig.1 sdAb stability improvement by introduction of disulfide bonds.¹

sdAb Stability Improvement Available at Creative Biolabs

With the amazing development of sdAb during the last few decades, it is necessary to extend the application of sdAbs in extreme environments against temperature-induced denaturation, pH-induced denaturation, protease hydrolysis, and other stress to achieve a variety of goals of research and investigation. To solve problems with insufficient stability and increase the widespread application of sdAbs, Creative Biolabs has developed a remarkable platform for sdAb stability improvement, which would be helpful for our customers' sdAb development projects.

Since sdAbs are composed of only one single-domain with a small size, their stability properties can be quickly and straightforwardly improved without impairing their binding properties to make them functional under relatively harsh conditions. Our scientists provide stability improvement by rational design of sdAbs or careful investigation of the suitable strategies, which may be generic solutions or be applied for specific sdAbs for our customers. Our services cover the improvement of both sdAb physical stability and chemical stability, including but not limited to:

sdAb Thermodynamic Stability Improvement
sdAb Proteolytic Tolerance Improvement

So far, multiple strategies are available to engineer sdAbs with enhanced stabilities at Creative Biolabs, which including but not limited to:

In general, one of these strategies to efficiently improve sdAbs stability can be used to make sure that the sdAb obtained is significantly more stable while having minimal effects on antigen-binding specificity and affinity. Through these strategies, stabilized sdAbs have been developed that retain their binding ability under extreme conditions, thereby providing superior reagents for a myriad of biotechnology, detection, diagnostic, and therapeutic applications.

Creative Biolabs is a pioneer and undisputed global leader who focuses on engineering-enhanced stability into sdAbs. As stability is one of the most important requirements in the application of therapeutic and diagnostic, Creative Biolabs is glad to provide sdAb stability improvement service for our clients all over the world. For more detailed information, please feel free to directly send us an inquiry, our scientists are pleased to work closely to promote your project.

Reference

1. Goldman, Ellen R., et al. "Enhancing stability of camelid and shark single domain antibodies: an overview." Frontiers in Immunology 8 (2017): 865. Distributed under Open Access license CC BY 4.0, without modification.

We are offering highly customized CRO services to assist your Single Domain Antibody (sdAb) related projects. Please Contact Us for more details.