SLC16A8 Membrane Protein Introduction

Introduction of SLC16A8

Solute carrier family 16 member 8 (SLC16A8), also known as monocarboxylate transporter 3 (MCT3), is encoded by the SLC16A8 gene. SLC16A8 is initially expressed in retinal pigment epithelial cells (RPE) in chickens and rats, and its subcellular localization is localized to the basement membrane of RPE. SLC16A8 is an ion co-transporter and requires normal biological function with the help of the accessory protein CD147. SLC16A8 is thought to play an important role in facilitating the transport of glycolytically derived lactic acid out of the retina.

Basic Information of SLC16A8
Protein Name	Monocarboxylate transporter 3
Gene Name	SLC16A8
Aliases	Solute carrier family 16 member 8
Organism	Homo sapiens (Human)
UniProt ID	O95907
Transmembrane Times	12
Length (aa)	504
Sequence	MGAGGPRRGEGPPDGGWGWVVLGACFVVTGFAYGFPKAVSVFFRALMRDFDAGYSDTAWVSSIMLAMLYGTGPVSSILVTRFGCRPVMLAGGLLASAGMILASFATRLLELYLTAGVLTGLGLALNFQPSLIMLGLYFERRRPLANGLAAAGSPVFLSALSPLGQQLLERFGWRGGFLLLGGLLLHCCACGAVMRPPPGPGPRPRRDSAGDRAGDAPGEAEADGAGLQLREASPRVRPRRRLLDLAVCTDRAFAVYAVTKFLMALGLFVPAILLVNYAKDAGVPDTDAAFLLSIVGFVDIVARPACGALAGLARLRPHVPYLFSLALLANGLTDLSSARARSYGALVAFCVAFGLSYGMVGALQFEVLMAAVGAPRFPSALGLVLLVEAAAVLIGPPSAGRLVDVLKNYEIIFYLAGSEVALAGVFMAVATNCCLRCAKAAPSGPGTEGGASDTEDAEAEGDSEPLPVVAEEPGNLEALEVLSARGEPTEPEIEARPRLAAESV

Function of SLC16A8 Membrane Protein

SLC16A8 is a proton-coupled monocarboxylate transporter preferentially expressed in the basolateral membrane of the retinal pigment epithelium (RPE) and has been shown to play an important role in regulating pH and lactate concentrations in the outer retina. Expression of SLC16A8 is confined to the RPE and choroid plexus epithelia where it is located on the basal membrane in contrast to SLC16A1 which is found on the apical membrane. It is documented that SLC16A8 plays an important role in facilitating the transport of glycolytically derived lactic acid out of the retina. Decreased expression of SLC16A8 in response to trauma or disease could contribute to pathologic changes in the retina. However, mice in which SLC16A8 was genetically deleted were healthy with no significant abnormalities in retinal histology.

SLC16A8 Membrane Protein Introduction Fig.1 Metabolic and redox signaling of the NXNL1 gene products. (Le´veillard, 2017)

Application of SLC16A8 Membrane Protein in Literature

Sharma K., et al. Serum Levels of TIMP-3, LIPC, IER3, and SLC16A8 in CFH-Negative AMD Cases. J Cell Biochem. 2017, 118(8): 2087-2095. PubMed ID: 27966779

The authors found that the expression level of LIPC, SLC16A8, and TIMP-3 were significantly associated with AMD pathology.
Li Y., et al. Effect of medium-chain triglycerides on growth performance, nutrient digestibility, plasma metabolites and antioxidant capacity in weanling pigs. Anim Nutr. 2015, 1(1): 12-18. PubMed ID: 29767040

Authors found that apparent total tract digestibility (ATTD) of ether extract was improved by MCT2 and MCT3 treatment from day 12-14 post-weaning. MCTs could improve growth performance, nutrients utilization, and antioxidant ability of weanling piglets.
Rahmatzadeh M., et al. A novel agent with histone deacetylase inhibitory activity attenuates neointimal hyperplasia. Cardiovasc Drugs Ther. 2014, 28(5): 395-406. PubMed ID: 25005755

MCT-3, with histone deacetylase inhibitory activity, was able to inhibit NIH and identify a potential molecular mechanism responsible for these effects.
Castorino J.J., et al. Basolateral sorting signals regulating tissue-specific polarity of heteromeric monocarboxylate transporters in epithelia. Traffic. 2011, 12(4): 483-498. PubMed ID: 21199217

This article showed that MCT3 and MCT4 contained strong redundant basolateral sorting signals (BLSS) in their C-terminal cytoplasmic tails that could direct fusion proteins with the apical marker p75 to the basolateral membrane.
Colombo S.G., et al. Modulation of MCT3 expression during wound healing of the retinal pigment epithelium. Invest Ophthalmol Vis Sci. 2010, 51(10): 5340-5350. PubMed ID: 20505202

This article revealed that wounding of RPE monolayers resulted in dedifferentiation of the cells at the edge of the wound, as evidenced by a loss of MCT3 and increased MCT4 expression.

SLC16A8 Preparation Options

To obtain the soluble and functional target protein, the versatile Magic™ membrane protein production platform in Creative Biolabs enables many flexible options, from which you can always find a better match for your particular project. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-SLC16A8 antibody development services.

Creative Biolabs' skillful scientists are glad to leverage our expertise and advanced technologies to help you with the member protein research. If you are interested, please feel free to contact us for more details.

Reference

Le´veillard Thierry. (2017). Metabolic and redox signaling in the retina. Cellular and Molecular Life Sciences. 74: 3649-3665.

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