SLC30A5 Membrane Protein Introduction

Introduction of SLC30A5

Zinc transporter 5 (ZnT-5), also known as carrier family 30 member 5 (SLC30A5), is a protein that in humans is encoded by the SLC30A5 gene. Zinc transporter 5 is a member of the SLC30A/ZnT family of zinc transporter proteins. ZnT proteins mediate both zinc sequestration into membrane-bound organelles and cellular zinc efflux. The encoded protein plays a role in the early secretory pathway as a heterodimer with zinc transporter 6 and may also regulate zinc sequestration into secretory granules of pancreatic beta cells. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome 19. Recent evidence indicates that the (predicted) cytosolic C-terminal tail of ZnT5 variant A is essential for heterodimerization with the ZnT6 zinc transporter. These studies suggest that the N-terminal region of ZnT5 is not involved in the interaction with ZnT6. This is based on co-localization with ZnT6 to a vesicular compartment of ZnT5 expressed with an N-terminal deletion.

Function of SLC30A5 Membrane Protein

Zinc plays a unique and important role in many key biological processes, such as growth, development, reproduction, and immune function. Therefore, maintaining an adequate but tightly regulated intracellular zinc level is critical for the survival of living organisms including humans. The SLC30 family is involved in 1) transferring zinc from the cytoplasm into intracellular compartments to provide zinc for zinc-containing proteins and store zinc in the cells; 2) shuffling cytoplasmic zinc out to the extracellular space to avoid zinc toxicity. SLC30A5 plays an important role in the maturation of osteoblasts and in the maintenance of the cells involved in the cardiac conduction system. It is associated with multiple diseases, including zinc-deficiency type, autosomal recessive 49, deafness, and acrodermatitis enteropathica.

Fig.1 Interacting Proteins for SLC30A5 Gene.

Application of SLC30A5 Membrane Protein in Literature

1. Petkovic V., et al. Alteration of ZnT5-mediated zinc import into the early secretory pathway affects the secretion of growth hormone from rat pituitary cells. Horm Res Paediatr. 2014. 82(4): 245-51. PubMed ID: 25196974
   
   

   The article reveals that ZnT5 might play an important role in regulated GH secretion. Altered expression of rSlc30a5 evokes changes in the cytoplasmic Zn²⁺ pool indicating its important role in mediating Zn²⁺ influx into intracellular compartments of the regulated secretory pathway.

2. Zhang X., et al. Zinc transporter 5 and zinc transporter 7 induced by high glucose protects peritoneal mesothelial cells from undergoing apoptosis. Cell Signal. 2013, 25(4): 999-1010. PubMed ID: 23275032
   
   

   Authors in this group demonstrate that the zinc transporting system in RPMCs is influenced by exposure to HG, particularly ZnT5 and ZnT7

3. Thornton J.K., et al. Differential subcellular localization of the splice variants of the zinc transporter ZnT5 is dictated by the different C-terminal regions. PLoS One. 2011, 6(8): e23878. PubMed ID: 21887337
   
   

   The article demonstrates that exons 15 to 17 include a signal that leads to trafficking of ZnT5 to the Golgi apparatus and that the 3' end of exon 14 includes a signal that leads to retention in the ER.

4. Nishida K., et al. Zinc transporter Znt5/Slc30a5 is required for the mast cell-mediated delayed-type allergic reaction but not the immediate-type reaction. J Exp Med. 2009, 206(6): 1351-64. PubMed ID: 19451265
   
   

   The article reveals that Znt5 is selectively required for the mast cell-mediated delayed-type allergic response. It is a novel participant in mast cell activation.

5. Inoue K., et al. Osteopenia and male-specific sudden cardiac death in mice lacking a zinc transporter gene, Znt5. Hum Mol Genet. 2002, 11(15): 1775-84. PubMed ID: 12095919
   
   

   This article reports that Znt5 protein plays an important role in the maturation of osteoblasts and in the maintenance of the cells involved in the cardiac conduction system.

SLC30A5 Preparation Options

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