5-HTR3B Membrane Protein Introduction

Introduction of 5-HTR3B

5-hydroxytryptamine receptor 3B (5-HTR3B), also known as serotonin receptor 3B, is one of the type 3 receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone which acts as a neurotransmitter, a hormone, and a mitogen. The 5-HTR3B receptor is a ligand-gated ion channel and in human is encoded by the HTR3B gene. The 5-HTR3B receptors are co-expressed with the 5-HTR3A subunit in the amygdala, hippocampus and caudate. They are also found in the kidney and testis as well as in monocytes of the spleen, small and large intestine, prostate, uterus, ovary, and placenta.

Basic Information of 5-HTR3B
Protein Name	5-hydroxytryptamine receptor 3B
Gene Name	HTR3B
Aliases	Serotonin receptor 3B, 5-HT3-B, 5-HT3B
Organism	Homo sapiens (Human)
UniProt ID	O95264
Transmembrane Times	4
Length (aa)	430
Sequence	MIVYFPGILATDTHHPQDSALYHLSKQLLQKYHKEVRPVYNWTKATTVYLDLFVHAILDVDAENQILKTSVWYQEVWNDEFLSWNSSMFDEIREISLPLSAIWAPDIIINEFVDIERYPDLPYVYVNSSGTIENYKPIQVVSACSLETYAFPFDVQNCSLTFKSILHTVEDVDLAFLRSPEDIQHDKKAFLNDSEWELLSVSSTYSILQSSAGGFAQIQFNVVMRRHPLVYVVSLLIPSIFLMLVDLGSFYLPPNCRARIVFKTSVLVGYTVFRVNMSNQVPRSVGSTPLIGHFFTICMAFLVLSLAKSIVLVKFLHDEQRGGQEQPFLCLRGDTDADRPRVEPRAQRAVVTESSLYGEHLAQPGTLKEVWSQLQSISNYLQTQDQTDQQEAEWLVLLSRFDRLLFQSYLFMLGIYTITLCSLWALWGGV

Function of 5-HTR3B Membrane Protein

5-hydroxytryptamine or 5-HTR, also known as the neurotransmitter serotonin receptor, mediates rapid excitatory responses through 5-HTR3 receptors (ligand-gated channels). The 5-HTR3B receptor is a cation-specific, but otherwise relatively nonselective ion channel, which causes fast, depolarizing responses upon activation. Activation of 5-HTR3 receptors generates a range of effects, such as membrane depolarization and increase in intracellular Ca²⁺, Ca²⁺ influx into lymphocytes, modulation of neurotransmitter release, excitation of central and peripheral neurons, and release of 5-HTR from enterochromaffin cells of the small intestine. Studies indicated that 5-HTR3B may play a role in tissue-specific functional changes of 5-HTR3-mediated signaling and/or modulation. Although 5-HTR3B seems to be unable to function as an α-subunit in homomers or in combination with nicotinic ACh receptor β-subunits, it is supposed to be involved in modifying 5-HTR3A receptor function and altering the desensitization of 5-HTR3A receptors.

Fig.1 Structure of mouse 5-HTR3B membrane protein.

Application of 5-HTR3B Membrane Protein in Literature

Farhat K., et al. Association of anti-emetic efficacy of Ondansetron with 18792A>G polymorphism in a drug target gene 5-HT3B in Pakistani population. J Pak Med Assoc. 2018, 68(5):733-736. PubMed ID: 29885172

The authors of this study focused on the evaluation of the association of anti-emetic efficacy of ondansetron with 18792A>G polymorphism in the target gene of 5-hydroxytryptamine type 3 subtype B.
Nakamura Y., et al. Yokukansan contains compounds that antagonize the 5-HT3 receptor. Phytomedicine. 2018, 43:120-125. PubMed ID: 29747744

The results attempted to evaluate the effect of seven alkaloids in Uncaria hook on the hydroxytryptamine type-3 (5-HT3) receptor ion channel and showed that six alkaloids weakly inhibited the 5-HT-mediated 5-HT3A and/or 5-HT3AB receptor current.
López-Morales P., et al. Genetic Factors Associated with Postoperative Nausea and Vomiting: a Systematic Review. J Gastrointest Surg. 2018, 22(9):1645-1651. PubMed ID: 29725907

The findings of this study indicated that the AAG deletion in both alleles of the 5-HT3B receptor gene appeared to be related to a higher incidence of PONV.
Nakamura Y., et al. Direct modification of the 5-HT3 receptor current by some anticancer drugs. Eur J Pharmacol. 2018, 821:21-28. PubMed ID: 29277715

Authors of this study suggested that over 25% of anticancer drugs tested in this study inhibited or potentiated the 5-HT3 receptor current.
Kim M.S., et al. Association of 5-HT3B Receptor Gene Polymorphisms with the Efficacy of Ondansetron for Postoperative Nausea and Vomiting. Yonsei Med J. 2015, 56(5):1415-20. PubMed ID: 26256989

The findings of this article suggested that the response to ondansetron for PONV was significantly influenced by the -100_-102AAG deletion polymorphisms of the 5-HT3B gene.

5-HTR3B Preparation Options

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