Muscle Targeting In Vivo Phage Library Screening Service

Background Muscle Targeting In Vivo Screening Platform FAQ Resources

Creative Biolabs offers clients a powerful service for isolating muscle targeting peptides by in vivo phage display method. These muscle targeting peptides have many potential applications for oligonucleotide or drug delivery to muscle for myopathies. Besides that, this method identifies muscle cell-surface receptors that can be used as potential targets for genetic modification of adenovirus tropism.

Background

Muscle, as an indispensable part of almost all animal species, makes up largest tissue volume of the body, while also yields several challenges and difficulties for muscle-specific therapy. Normal life activities, such as respiration, ambulation and cardiac function, are mainly related to a series of muscles including skeletal, cardiac and laryngeal muscles. However, a large group of inherited or acquired myopathies are existing worldwide and affecting the ability of muscles to exert their function for both adults and children. At present, gene therapy shows particularly attraction for the treatment of various inherited myopathies, such as muscular dystrophies and heart failure. Ideally, the therapy should be carried out through specifically targeted vector construct or pharmaceuticals to muscles following intravenous injection. But up to now the targeting mechanisms for mature skeletal or cardiac muscle are still absence. Thus, the identification of muscle targeting moieties (e.g. muscle-specific peptides) is meaningful and valuable. One particular choice is applying in vivo phage display, which can provide high specificity and diversity candidates to reduce the oligonucleotide dosage of therapy or minimize the possibility of non-muscle toxicity.

Muscle Targeting In Vivo Phage Library Screening Platform

Innovative scientists of Creative Biolabs have developed a novel in vivo phage display platform for selecting tissue-specific peptides. Using this method, a great deal of peptides or protein ligands candidates, which is specific for the target muscle tissues, can be isolated form cell surface. Those selected peptides will contain fully in vivo characteristics and can be used for transporting therapeutic agents to muscles and improve drug specificity and efficacy. In addition, it is also possible to identify some novel muscle cell-surface receptors by this method and then provides important tools for improving the efficiency of gene transfer in mammalian muscles, such as developing targeted adenoviral vectors.

Creative Biolabs has been a long-term expert in the field of phage display. Our most devoted scientists are confident in offering our customers the best service to meet all individual required specifications.

Muscle targeting in vivo phage library screening. (Creative Biolabs Original) Fig 1. Screening of muscle-targeting peptides.

Other optional in vivo phage display screening services:

Reference

Seow, Y., Yin, H. and Wood, M. J. (2010) 'Identification of a novel muscle targeting peptide in mdx mice', Peptides, 31(10), 1873-1877.

FAQ

What is muscle-targeting in vivo phage display library screening?
Muscle-targeting in vivo phage display library screening is a specialized technique used to identify peptides or proteins that specifically bind to muscle tissues. This method involves the administration of phage display libraries into living organisms and isolating phages that bind selectively to muscle cells, helping in the development of targeted therapies for muscular disorders.
Why is muscle-targeting important in medical research?
Muscle-targeting is crucial for developing treatments for a variety of muscular disorders, including muscular dystrophies, atrophy, and inflammation-related muscle diseases. Targeting specific muscle tissues can enhance the delivery and efficacy of therapeutic agents, potentially reducing side effects associated with systemic treatments and improving patient outcomes.
What are the common applications of muscle-targeting phage display?
Common applications include the development of targeted drug delivery systems for muscle regeneration, therapies for muscle-specific diseases, and diagnostic tools for detecting muscle damage or disease. Additionally, this technique can be used to study muscle biology and discover new biomarkers for muscular disorders.
What types of peptides or proteins are typically identified in muscle-targeting phage display?
The peptides or proteins identified are often those that bind to specific receptors or structural components unique to muscle cells, such as dystrophin, myosin, or muscle-specific integrins. These molecules can serve as delivery vehicles for drugs or as part of therapeutic agents themselves.
How does muscle-targeting in vivo phage display differ from other tissue-targeting methods?
Muscle-targeting focuses on the unique environment and cellular composition of muscle tissues, which differ significantly from other tissues due to their contractile nature and regeneration capacity. This specificity requires careful consideration of the physiological and biochemical characteristics of muscle tissues during the screening process.