ABCB1 Membrane Protein Introduction

Introduction of ABCB1

ABCB1 encoded by ABCB1 gene is a member of the superfamily of ATP-binding cassette (ABC) transporters which transport various molecules across extra- and intracellular membranes. There are seven subfamilies in ABC superfamily named as ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White, respectively. ABCB1 protein belongs to MRP subfamily with a typical structure of transporters. It possesses 12 transmembrane domains forming a channel pore and a nucleotide-binding domain for ATP-dependent translocation of substrates across the cell membrane.

Basic Information of ABCB1
Protein Name	Multidrug resistance protein 1
Gene Name	ABCB1
Aliases	CLCS, MDR1, P-GP, PGY1, ABC20, CD243, GP170
Organism	Homo sapiens (Human)
UniProt ID	P08183
Transmembrane Times	12
Length (aa)	1280
Sequence	MDLEGDRNGGAKKKNFFKLNNKSEKDKKEKKPTVSVFSMFRYSNWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFANAGNLEDLMSNITNRSDINDTGFFMNLEEDMTRYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQIHKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGEYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTAGNEVELENAADESKSEIDALEMSSNDSRSSLIRKRSTRRSVRGSQAQDRKLSTKEALDESIPPVSFWRIMKLNLTEWPYFVVGVFCAIINGGLQPAFAIIFSKIIGVFTRIDDPETKRQNSNLFSLLFLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAHKLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPNTLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQAGTKRQ

Function of ABCB1 Membrane Protein

ABCB1 is an ATP-dependent drug efflux pump that is responsible for transport endo- and xenobiotic compounds from cells into the extracellular spaces or out of the body. Hence, it plays an important role in gastrointestinal absorption, distribution, metabolism, excretion, and toxicity of its substrates. ABCB1 in the intestinal epithelium transport xenobiotics (such as toxins or drugs) back into the intestinal lumen. In liver cells, ABCB1 pumps xenobiotics into bile ducts. In the cells of the proximal tubule of the kidney, ABCB1 pumps xenobiotics into urinary filtrate (in the proximal tubule). In the capillary endothelial cells composing the blood-brain barrier and blood-testis barrier, ABCB1 pumps xenobiotics back into the capillaries. Moreover, ABCB1 expressed in the adrenal cortex has been revealed a role in hormone traffic and homeostasis. ABCB1 may also be involved in the transport of cytokines and enveloped viruses in lymphocytes and lipid homeostasis in the periphery and central nervous system. In addition, some studies have shown that ABCB1 is associated with the progression of cancer and the overexpression of ABCB1 is observed in the numerous tissues transformed by cancer.

Fig.1 Structure of the mouse P-glycoprotein based on the 3g5u PDB coordinates.

Application of ABCB1 Membrane Protein in Literature

Allegra S., et al. A common mdr1 gene polymorphism is associated with changes in linezolid clearance. Ther Drug Monit. 2018, 40(5): 602-609. PubMed ID: 29979333

The study reveals a polymorphism c.3435C>T in ABCB1 is associated with linezolid pharmacokinetics, suggesting a new sight for pharmacogenetic analyses in antimicrobial chemotherapy.
Hammad A.M., et al. Alcohol and cocaine exposure modulates ABCB1 and ABCG2 transporters in male alcohol-preferring rats. Mol Neurobiol. 2018. PubMed ID: 29978425

The study shows that the expression levels of ABCB1 and ABCG2 are changed in the rat brain area when rats are chronically exposed to commonly abused drugs, ethanol, and cocaine.
Alasmari F., et al. Modulation of the ATP-Binding cassette B1 transporter by neuro-inflammatory cytokines: role in the pathogenesis of Alzheimer's Disease. Front Pharmacol, 2018, 9: 658. PubMed ID: 29973883

The study shows that the decreased expression of ABCB1 is associated with the accumulation of Aβ which lead to a decreased level of pro-inflammatory cytokines in microglia. This suggests a role of ABCB1 in the pathogenesis of Alzheimer's Disease.
Wang R., et al. Long non-coding RNA FTH1P3 activates paclitaxel resistance in breast cancer through miR-206/ABCB1. J Cell Mol Med, 2018, 22(9): 4068-4075. PubMed ID: 29971911

The study reveals that lncRNA ferritin heavy chain 1 pseudogene 3 (FTH1P3) regulates breast cancer paclitaxel resistance through miR-206/ABCB1, which helps to explain the breast cancer chemoresistance.
Zhou T., et al. ABCA1 is coordinated with ABCB1 in the arsenic-resistance of human cells. Appl Biochem Biotechnol, 2018. PubMed ID: 29951962

The study shows that ABCA1 coordinated with ABCB1 promotes arsenic resistance in arsenic-resistant ECV-304 (AsRE) cell line.

ABCB1 Preparation Options

To obtain the soluble and functional target protein, the versatile Magic™ membrane protein production platform in Creative Biolabs enables many flexible options, from which you can always find a better match for your particular project. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-ABCB1 antibody development services.

As a forward-looking research institute as well as a leading custom service provider in the field of membrane protein, Creative Biolabs has won good reputation among our worldwide customers for successfully accomplishing numerous challenging projects including generation of many functional membrane proteins. Please feel free to contact us for more information.

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