ABCC1 Membrane Protein Introduction

Introduction of ABCC1

ABCC1, also known as MRP1, is a member of the superfamily of ATP-binding cassette (ABC) transporters which transport various molecules across extra- and intracellular membranes. There are seven subfamilies in ABC superfamily named as ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White, respectively. ABCC1 protein belongs to MRP subfamily. It is a 190 kDa protein with three membrane-spanning domains that contains 17 transmembrane helixes, and two nucleotide binding domains. ABCC1 is expressed in most human tissues, especially in lungs, kidneys, placenta, bladder, and adrenal glands, even in the endothelium cells of the blood-brain barrier.

Function of ABCC1 Membrane Protein

ABCC1 has been revealed to exert an essential role in the regulation of cellular oxidative stress and redox homeostasis. It is a typical ATP-dependent transporter of multispecific organic anions with a high affinity for glutathione (GSH) conjugates. ABCC1 also transports leukotriene C4 (LTC4), estradiol-17β-glucuronate, sulfated bile acids, bilirubin, and folate. Moreover, ABCC1 has been also suggested to be a transporter for glucuronides and sulfate conjugates of steroid hormones and bile salts. Recently, some studies reported that ABCC1 is most present at the blood-brain barrier and plays a role in the protection of the brain from xenobiotics along with P-gp and BCRP. And ABCC1 can decrease β-amyloid accumulation and involves in the progression of Alzheimer’s disease. In addition, ABCC1 has been revealed to be responsible for multiple drugs resistance such as anthracyclines, camptothecins, vinca alkaloids, a few kinase inhibitors, etoposide, irinotecan, SN-38, and methotrexate. The overexpression of ABCC1 is found in many tumor tissues and involves in the resistance of chemotherapeutic drugs thereby impacting the therapeutic process. In lung carcinoma, higher expression of ABCC1 is associated with a decreased sensibility to chemotherapeutic drugs and survival rate.

Fig.1 Structure of ABCC1 protein.

Application of ABCC1 Membrane Protein in Literature

1. Weigl K.E., et al. An outward-facing a romatic amino acid is crucial for signaling between the membrane spanning and nucleotide binding domains of multidrug resistance protein 1 (MRP1; ABCC1). Mol Pharmacol. 2018, 94(3): 1069-1078. PubMed ID: 29976562.
   
   

   The study shows that the Phe583 side-chain of ABCC1 is very important for its transport function. The loss of F583A Phe583 side-chain can alter the solute binding and nucleotide binding regions of the transporter, thereby making ABCC1 in a low-affinity solute binding state.

2. Hu H., et al. Long non-coding RNA KCNQ1OT1 modulates oxaliplatin resistance in hepatocellular carcinoma through miR-7-5p/ ABCC1 axis. Biochem Biophys Res Commun. 2018, 503(4): 2400-2406. PubMed ID: 29966655
   
   

   The study reveals that long non-coding RNA KCNQ1OT1 participates in the regulation of oxaliplatin resistance in hepatocellular carcinoma cells through miR-7-5p/ABCC1 pathway, which suggests a potential therapeutic strategy for hepatocellular carcinoma.

3. Li Y., et al. miR-1268a regulates ABCC1 expression to mediate temozolomide resistance in glioblastoma. Journal of neuro-oncology. 2018. PubMed ID: 29876787
   
   

   The study indicates that miR-1268a regulates temozolomide resistance in glioblastoma through controlling the expression of ABCC1.

4. Banerjee M., et al. Multidrug Resistance Protein 1 (MRP1/ABCC1)-mediated cellular protection and transport of methylated arsenic metabolites differs between human cell lines. Drug Metabolism & Disposition the Biological Fate of Chemicals. 2018, 46(8): 1096-1105. PubMed ID: 29752257
   
   

   The study shows that multiple factors including cell and tissue type influence the efflux of different arsenic metabolites by MRP1. This suggests the effect of MRP1 on both tissue-specific risks to arsenic-induced disease and tumor sensitivity to arsenic-based therapeutics.

5. Zukauskas A., et al. Transporters MRP1 and MRP2 regulate opposing inflammatory signals to control transepithelial neutrophil migration during streptococcus pneumoniae lung infection. mSphere. 2018, 3(4). pii: e00303-18. PubMed ID: 29976647
   
   

   The study reveals that transporters MRP1 and MRP2 play an important role in the neutrophil control in the lung. This suggests the potential therapeutic targets to decrease excess inflammation, thereby reducing the susceptibility of developing bacteremia during pneumococcal pneumonia.

ABCC1 Preparation Options

To obtain the soluble and functional target protein, the versatile Magic™ membrane protein production platform in Creative Biolabs enables many flexible options, from which you can always find a better match for your particular project. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-ABCC1 antibody development services.

• Magic™ Membrane Protein Production Platform
• Magic™ Anti-Membrane Protein Antibody Discovery Platform

As a forward-looking research institute as well as a leading custom service provider in the field of membrane protein, Creative Biolabs has won good reputation among our worldwide customers for successfully accomplishing numerous challenging projects including generation of many functional membrane proteins. Please feel free to contact us for more information.

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