What is anti-MIgG(Fc)Fab-N-MMAF ADC?

Anti-MIgG(Fc)Fab-N-MMAF ADC is an antibody-drug conjugate consisting of a Fab fragment of an anti-mouse IgG Fc specific polyclonal antibody linked via a noncleavable linker to the cytotoxic molecule MMAF. It is used for pre-screening mouse monoclonal antibodies as ADC candidates.

How does the Fab fragment function in anti-MIgG(Fc)Fab-N-MMAF ADC?

The Fab fragment of the antibody specifically targets the Fc region of mouse IgG. This fragment is smaller than a full antibody, which can enhance tissue penetration and reduce immunogenicity while still delivering the cytotoxic MMAF to the target cells.

What are the benefits of using a noncleavable linker in anti-MIgG(Fc)Fab-N-MMAF ADC?

The use of a noncleavable linker in this ADC ensures that the cytotoxic drug MMAF remains attached to the antibody fragment until it reaches the target site. This can enhance the stability and reduce the potential off-target effects of the drug.

How does anti-MIgG(Fc)Fab-N-MMAF ADC compare to traditional full antibody ADCs?

This ADC, using a Fab fragment instead of a full antibody, offers advantages such as better tissue penetration and lower immunogenicity. These properties can make it more effective in certain research applications compared to traditional full antibody ADCs.

What is the significance of the Fc region in the context of anti-MIgG(Fc)Fab-N-MMAF ADC?

The Fc region of IgG is critical for mediating various immune responses. In this ADC, the Fab fragment targets this region, facilitating the delivery of MMAF to the intended site while leveraging the conserved N-glycosylation site essential for Fc receptor-mediated activity.

What are the potential research applications of anti-MIgG(Fc)Fab-N-MMAF ADC?

This ADC is used for pre-screening mouse monoclonal antibodies, optimizing ADC formulations, and studying ADC mechanisms. Its unique composition allows researchers to evaluate the cytotoxic effects of MMAF in different experimental setups.

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anti-MIgG(Fc)Fab-N-MMAF ADC

anti-MIgG(Fc)Fab-N-MMAF ADC (CAT#: ADC-AA-027)

This ADC product is comprised of a Fab fragment of an anti-mouse IgG Fc specific polyclonal antibody conjugated via a noncleavable linker to MMAF. The antibody portion is a Fab fragment of a secondary antibody and the drug portion, MMAF, is a cytotoxic small molecule which binds to tubulins, interrupts microtubule dynamics, and induces cell death. This product displays no obvious toxicity without a primary antibody and can be a quite efficient and economical alternative to pre-screening mouse monoclonal antibodies as ADC candidates.

ADC Target
ADC Antibody
ADC Linker
ADC payload drug

Name
IgG Fc

Overview
The fragment crystallizable region (Fc region) is composed of the constant region of the two heavy chains that form the IgG molecule. The Fc region of IgG bears a highly conserved N-glycosylation site. Glycosylation of the Fc fragment is essential for Fc receptor-mediated activity. Fc binds to various cell receptors and complement proteins thus mediating different physiological effects of antibodies, such as opsonization, antibody dependent cellular cytotoxicity (ADCC), degranulation of mast cells, basophils, eosinophils and other processes.

Overview
Fab fragment of anti-mouse IgG Fc specific polyclonal antibody

Species Reactivity
Mouse

Name
Noncleavable linkers

Description
Noncleavable linkers, is considered noncleavable-meaning linker cleavage, and payload release does not depend on the differential properties between the plasma and some cytoplasmic compartments. Instead, the release of the cytotoxic drug is postulated to occur after internalization of the ADC via antigen-mediated endocytosis and delivery to lysosomal compartment, where the antibody is degraded to the level of amino acids through intracellular proteolytic degradation.

Name
MMAF (Monomethyl auristatin F)

Description
Derived from Auristatin,are water-soluble dolastatin analogs of dolastatin 10. Dolastatin 10 belongs to dolastatin family and it can powerfully bind to tubulin, thus inhibiting polymerization mediated through the binding to the vinca alkaloid binding domain, and causes cell to accumulate in metaphase arrest.

For Research Use Only. NOT FOR CLINICAL USE.

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Customer Reviews FAQ

Excellent

The anti-MIgG(Fc)Fab-N-MMAF ADC has been incredibly effective at inducing cell death in our target models without affecting surrounding cells. This specificity is crucial for our research.

Excellent

We've found the anti-MIgG(Fc)Fab-N-MMAF ADC exceptionally reliable for studying microtubule dynamics. The MMAF binds effectively, disrupting cell function leading to apoptosis.

Excellent

I recommend this ADC for anyone looking into detailed, accurate pre-screening of mouse monoclonal antibodies. Its cost-efficiency and precision make it invaluable.

Excellent

The stability and cell-targeting precision of the anti-MIgG(Fc)Fab-N-MMAF ADC have significantly accelerated our antibody screening projects. It's a powerful tool for advanced research.

Excellent

Using the anti-MIgG(Fc)Fab-N-MMAF ADC has transformed our ADC candidate evaluations. The noncleavable linker provides continuous delivery of MMAF, increasing the cytotoxic effectiveness.

Excellent

The specificity and reduced toxicity of the anti-MIgG(Fc)Fab-N-MMAF ADC make it a prime choice for our lab's focus on reducing adverse effects while maintaining therapeutic efficacy.

Q: 1: What is anti-MIgG(Fc)Fab-N-MMAF ADC?
A: Anti-MIgG(Fc)Fab-N-MMAF ADC is an antibody-drug conjugate consisting of a Fab fragment of an anti-mouse IgG Fc specific polyclonal antibody linked via a noncleavable linker to the cytotoxic molecule MMAF. It is used for pre-screening mouse monoclonal antibodies as ADC candidates.
Q: 2: How does the Fab fragment function in anti-MIgG(Fc)Fab-N-MMAF ADC?
A: The Fab fragment of the antibody specifically targets the Fc region of mouse IgG. This fragment is smaller than a full antibody, which can enhance tissue penetration and reduce immunogenicity while still delivering the cytotoxic MMAF to the target cells.
Q: 3: What are the benefits of using a noncleavable linker in anti-MIgG(Fc)Fab-N-MMAF ADC?
A: The use of a noncleavable linker in this ADC ensures that the cytotoxic drug MMAF remains attached to the antibody fragment until it reaches the target site. This can enhance the stability and reduce the potential off-target effects of the drug.
Q: 4: How does anti-MIgG(Fc)Fab-N-MMAF ADC compare to traditional full antibody ADCs?
A: This ADC, using a Fab fragment instead of a full antibody, offers advantages such as better tissue penetration and lower immunogenicity. These properties can make it more effective in certain research applications compared to traditional full antibody ADCs.
Q: 5: What is the significance of the Fc region in the context of anti-MIgG(Fc)Fab-N-MMAF ADC?
A: The Fc region of IgG is critical for mediating various immune responses. In this ADC, the Fab fragment targets this region, facilitating the delivery of MMAF to the intended site while leveraging the conserved N-glycosylation site essential for Fc receptor-mediated activity.
Q: 6: What are the potential research applications of anti-MIgG(Fc)Fab-N-MMAF ADC?
A: This ADC is used for pre-screening mouse monoclonal antibodies, optimizing ADC formulations, and studying ADC mechanisms. Its unique composition allows researchers to evaluate the cytotoxic effects of MMAF in different experimental setups.

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Same Target Same Linker Same Payload

CAT#	Product Name	Linker	Payload
ADC-AA-020	anti-MIgG(Fc)-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-026	anti-MIgG(Fc)-N-DM1 ADC	Noncleavable linkers	DM1 (N2’-Deacetyl-N2’-(3-mercapto-1-oxopropyl)maytansine)
ADC-AA-029	anti-MIgG(Fc)Fab-C-MMAE ADC	Cleavable linkers	MMAE (Monomethyl auristatin E)
ADC-AA-049	Protein G-MMAF ADC		MMAF (Monomethyl auristatin F)
ADC-AA-031	anti-MIgG(Fc)Fab-N-DM1 ADC	Noncleavable linkers	DM1 (N2’-Deacetyl-N2’-(3-mercapto-1-oxopropyl)maytansine)

CAT#	Product Name	Linker	Payload
ADC-AA-034	anti-RIgG(HL)-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-008	anti-HIgG(Fc)-N-DM1 ADC	Noncleavable linkers	DM1 (N2’-Deacetyl-N2’-(3-mercapto-1-oxopropyl)maytansine)
ADC-AA-041	anti-Rat IgG(Fc)Fab-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-046	anti-Rat IgG(HL)Fab-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-045	anti-Rat IgG(HL)-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)

CAT#	Product Name	Linker	Payload
ADC-AA-020	anti-MIgG(Fc)-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-W-485	Anti-EphA2 (clone 1C1)-Mc-MMAF ADC	Mc (maleimidocaproyl)	MMAF (Monomethyl auristatin F)
ADC-AA-002	anti-HIgG(Fc)-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-049	Protein G-MMAF ADC		MMAF (Monomethyl auristatin F)
ADC-AA-053	Protein A-MMAF ADC		MMAF (Monomethyl auristatin F)

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