What is the recommended procedure for conjugating Protein A with MMAF?

We recommend following standardized conjugation protocols, optimizing reaction conditions based on specific experimental needs, and consulting detailed guidelines provided with the product.

Can this ADC product be used for targeting other cell types besides cancer cells?

Yes, while it is primarily designed for cancer cells, it can potentially target other cells that express the appropriate receptors.

What advantages does MMAF have over other cytotoxic agents in ADCs?

MMAF is highly potent, disrupts microtubule dynamics effectively, and has a proven track record in inducing cell death in cancer cells.

How should the ADC product be prepared for in vitro experiments?

Thaw the ADC slowly on ice, gently mix before use, and avoid vigorous shaking to maintain its integrity.

What is the shelf life of Protein A-MMAF ADC?

The product has a shelf life of up to one year when stored under recommended conditions (-80°C).

What are the potential side effects of using Protein A-MMAF ADC in preclinical studies?

While designed to minimize off-target effects, potential side effects may include localized toxicity depending on the experimental model and dosage used.

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Protein A-MMAF ADC

Protein A-MMAF ADC (CAT#: ADC-AA-053)

This ADC product is comprised of a Protein A conjugated via a linker to MMAF. The MMAF is targeted to certain cancers by immunerecognition and delivered into cancer cells via receptor mediated endocytosis. Within the cell, MMAF binds to tubulins, interrupts microtubule dynamics, and subsequently, induces cell death. This product displays no obvious toxicity without a primary antibody and can be a quite efficient and economical alternative to pre-screening monoclonal antibodies as ADC candidates.

ADC Target
ADC Antibody
ADC payload drug

Name
IgG Fc

Overview
The fragment crystallizable region (Fc region) is composed of the constant region of the two heavy chains that form the IgG molecule. The Fc region of IgG bears a highly conserved N-glycosylation site. Glycosylation of the Fc fragment is essential for Fc receptor-mediated activity. Fc binds to various cell receptors and complement proteins thus mediating different physiological effects of antibodies, such as opsonization, antibody dependent cellular cytotoxicity (ADCC), degranulation of mast cells, basophils, eosinophils and other processes.

Overview
Protein A

Name
MMAF (Monomethyl auristatin F)

Description
Derived from Auristatin,are water-soluble dolastatin analogs of dolastatin 10. Dolastatin 10 belongs to dolastatin family and it can powerfully bind to tubulin, thus inhibiting polymerization mediated through the binding to the vinca alkaloid binding domain, and causes cell to accumulate in metaphase arrest.

For Research Use Only. NOT FOR CLINICAL USE.

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Customer Reviews and FAQs

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Customer Reviews FAQ

Excellent

This ADC product is a cost-effective alternative for pre-screening monoclonal antibodies. The lack of toxicity without a primary antibody is a significant benefit.

Excellent

We've achieved excellent results with Protein A-MMAF ADC. Its ability to disrupt microtubule dynamics and induce cell death in cancer cells is impressive.

Excellent

Very satisfied

The targeted delivery of MMAF into cancer cells via receptor-mediated endocytosis makes this product incredibly effective for our studies.

Excellent

Using Protein A-MMAF ADC has significantly advanced our research. The precise targeting and efficient delivery system are remarkable.

Excellent

Great product

This ADC product from Creative Biolabs shows no toxicity without a primary antibody and provides an economical solution for our research needs.

Excellent

The conjugation of Protein A to MMAF works perfectly for our experiments. It's efficient, reliable, and has greatly contributed to our cancer research progress.

Q: 1: What is the recommended procedure for conjugating Protein A with MMAF?
A: We recommend following standardized conjugation protocols, optimizing reaction conditions based on specific experimental needs, and consulting detailed guidelines provided with the product.
Q: 2: Can this ADC product be used for targeting other cell types besides cancer cells?
A: Yes, while it is primarily designed for cancer cells, it can potentially target other cells that express the appropriate receptors.
Q: 3: What advantages does MMAF have over other cytotoxic agents in ADCs?
A: MMAF is highly potent, disrupts microtubule dynamics effectively, and has a proven track record in inducing cell death in cancer cells.
Q: 4: How should the ADC product be prepared for in vitro experiments?
A: Thaw the ADC slowly on ice, gently mix before use, and avoid vigorous shaking to maintain its integrity.
Q: 5: What is the shelf life of Protein A-MMAF ADC?
A: The product has a shelf life of up to one year when stored under recommended conditions (-80°C).
Q: 6: What are the potential side effects of using Protein A-MMAF ADC in preclinical studies?
A: While designed to minimize off-target effects, potential side effects may include localized toxicity depending on the experimental model and dosage used.

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Same Target Same Payload

CAT#	Product Name	Linker	Payload
ADC-AA-056	Anti-MIgG (clone 187.1)-VC-MMAE ADC	VC (valine-citrulline)	MMAE (Monomethyl auristatin E)
ADC-AA-055	Protein A-Duocarmycin ADC		Duocarmycins
ADC-AA-021	anti-MIgG(Fc)-C-MMAE ADC	Cleavable linkers	MMAE (Monomethyl auristatin E)
ADC-AA-026	anti-MIgG(Fc)-N-DM1 ADC	Noncleavable linkers	DM1 (N2’-Deacetyl-N2’-(3-mercapto-1-oxopropyl)maytansine)
ADC-AA-027	anti-MIgG(Fc)Fab-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)

CAT#	Product Name	Linker	Payload
ADC-W-537	Anti-TPBG-Mc-MMAF ADC	Mc (maleimidocaproyl)	MMAF (Monomethyl auristatin F)
ADC-AA-019	anti-MIgG(Fc)-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-010	anti-HIgG(Fc)Fab-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-W-491	Anti-TNFRSF17-Mc-MMAF ADC	Mc (maleimidocaproyl)	MMAF (Monomethyl auristatin F)
ADC-AA-020	anti-MIgG(Fc)-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)

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