Anti-CEA (8019) h(41BB-CD3ζ) CAR, pCDCAR1 vector

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Details

Target
CEA

Targeting Cell Type
T cell

Targeting Diseases
Colorectal cancer

Generation
Second

Vector Name
pCDCAR1

Vector Length
~8kb

Vector Type
Lentiviral vector

Receptor Construction
scFv-41BB-CD3ζ

Discription of Signaling Cassetes
41BB
CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. On the basis of preclinical observation, this molecule can promote the persistence of antigen-specific and antigennonspecific chimeric antigen receptor T-cells to significantly increases antitumor activity.
CD3ζ
CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

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Published Data

CAR scFv data RIA

Fig.1 Inhibition of Abl (8019) binding to Ab2 (3H1) on the plate by patients' Ab3 sera by radioimmunoassay.

CAR Construction :

Fig.1 Inhibition of Abl (8019) binding to Ab2 (3H1) on the plate by patients' Ab3 sera by radioimmunoassay.

Purified 3H1 was used to coat the plate (500 ng/well) .

Foon, K. A., Chakraborty, M., John, W. J., Sherratt, A., Köhler, H., & Bhattacharya-Chatterjee, M. (1995). Immune response to the carcinoembryonic antigen in patients treated with an anti-idiotype antibody vaccine. The Journal of clinical investigation, 96(1), 334-342.

CAR scFv data FuncS

Fig.2 Flow microfluorimetry analysis of the CEA positive colon cancer cell line, LS174-T, with patients' Ab3 sera.

CAR Construction :

Fig.2 Flow microfluorimetry analysis of the CEA positive colon cancer cell line, LS174-T, with patients' Ab3 sera.

Tumor cells were reacted with Ab3 sera (1/100 dilution) from patients immunized with murine Abl 8019.

CAR scFv data ELISA

Fig.3 ELISA analysis of the inhibition of biotinytated anti C3d-3H1 antibodies to Fab of 3H1 by Mab 8019

CAR Construction :

Fig.3 ELISA analysis of the inhibition of biotinytated anti C3d-3H1 antibodies to Fab of 3H1 by Mab 8019

Fab 3H1 coated plates and mouse anti-3H1-16mer serum were incubated in the presence of 8019 or OKT3.

Lou, D., & Kohler, H. (1998). Enhanced molecular mimicry of CEA using photoaffinity crosslinked C3d peptide. Nature biotechnology, 16(5), 458-462.

CAR scFv data IF

Fig.4 Immunofluorescence analysis of CEA-positive MC-38-CEA cells.

CAR Construction :

Fig.4 Immunofluorescence analysis of CEA-positive MC-38-CEA cells.

mAb 8019 was used as a positive control.

Baral, R., Saha, A., Chatterjee, S. K., Foon, K. A., Krieg, A. M., Weiner, G. J., & Bhattacharya-Chatterjee, M. (2003). Immunostimulatory CpG oligonucleotides enhance the immune response of anti-idiotype vaccine that mimics carcinoembryonic antigen. Cancer Immunology, Immunotherapy, 52(5), 317.

CAR scFv data Inhibit

Fig.5 Ab1 (mAb 8019) binding inhibition to Ab2 (3H1) by immune sera.

CAR Construction :

Fig.5 Ab1 (mAb 8019) binding inhibition to Ab2 (3H1) by immune sera.

Induction of anti-anti-idiotype antibody (Ab3) in mice after multiple immunizations with 3H1-CpG or 11D10-CpG.

More Published Data More Published Data

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Anti-CEA (8019) h(41BB-CD3ζ) CAR, pCDCAR1 (CAR-ZP4940)

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