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Anti-EGFR (5G9B5) h(CD28-CD3ζ) CAR, pCDCAR1 (CAR-WFY7361)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The vector of anti-EGFR chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target Human EGFR. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-EGFR antibody linked to CD28 and CD3ζ signaling domains. And the vector product was designed for the treatment of multiple myeloma.

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Details

  • Target
  • EGFR
  • Targeting Cell Type
  • T cell
  • Targeting Diseases
  • Plasma membrane
  • Generation
  • Second
  • Vector Name
  • pCDCAR1
  • Vector Length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Receptor Construction
  • scFv-CD28-CD3ζ
  • Discription of Signaling Cassetes
  • CD28
    CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. CD28 is the receptor for CD80 (B7.2) proteins which are expressed on antigen-presenting cells (APC). CD28 modulates the primary TCR/CD3ζ signal in a different fashion than the late costimulatory elements OX40 and 4-1BB. CD28 enhances the expression of downstream regulators that impact on T-cell proliferation, death, differentiation, and effector functions. CAR+ T cells containing the CD28 endodomain showed strikingly enhanced T cell activation, growth, survival. And CD28 results in a brightly expressed, stable receptor as the transmembrane domain. Including CD28 costimulatory domains in CARs led to enhanced anti-malignancy efficacy.
    CD3ζ
    CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ, ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMS, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound.CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Target

  • Clone
  • 5G9B5
  • Host
  • Mouse
  • Target Species
  • Human
  • Gene Name
  • Epidermal Growth Factor Receptor
  • Synonyms
  • EGFR;EGFR; Epidermal Growth Factor Receptor; Epidermal Growth Factor Receptor; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; ERBB1; ERBB; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian);

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  • Published Data
CAR scFv data FCM

Fig.1 FACS analysis on PTC cell lines stained with anti-EGFR Ab (dark black peak), with a mouse IgG1 isotype control (grey dotted peak) or only with the secondary labelled Ab (black peak).

CAR Construction : Latest CAR Construction

Fig.1 FACS analysis on PTC cell lines stained with anti-EGFR Ab (dark black peak), with a mouse IgG1 isotype control (grey dotted peak) or only with the secondary labelled Ab (black peak).

One representative experiment of two is shown.

Degl'Innocenti, D., Alberti, C., Castellano, G., Greco, A., Miranda, C., Pierotti, M. A., ... & Tomassetti, A. (2010). Integrated ligand-receptor bioinformatic and in vitro functional analysis identifies active TGFA/EGFR signaling loop in papillary thyroid carcinomas. PLoS One, 5(9), e12701.
CAR scFv data ELISA

Fig.2 TGFA production by thyroid cell lines.

CAR Construction : Latest CAR Construction

Fig.2 TGFA production by thyroid cell lines.

Media without FBS were conditioned for 48 h by confluent cells and analyzed by ELISA.

Degl'Innocenti, D., Alberti, C., Castellano, G., Greco, A., Miranda, C., Pierotti, M. A., ... & Tomassetti, A. (2010). Integrated ligand-receptor bioinformatic and in vitro functional analysis identifies active TGFA/EGFR signaling loop in papillary thyroid carcinomas. PLoS One, 5(9), e12701.
CAR scFv data FCM

Fig.3 FBP expression on IGROV1 cells and their cisplatin-resistant variants IGROV1/Pt 0.5 and IGROV1/Pt.

CAR Construction : Latest CAR Construction

Fig.3 FBP expression on IGROV1 cells and their cisplatin-resistant variants IGROV1/Pt 0.5 and IGROV1/Pt.

All measurements were performed in triplicate.

Ottone, F., Miotti, S., Bottini, C., Bagnoli, M., Perego, P., Colnaghi, M. I., & Ménard, S. (1997). Relationship between folate-binding protein expression and cisplatin sensitivity in ovarian carcinoma cell lines. British Journal of Cancer, 76(1), 77-82.

More Published Data More Published Data

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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