Anti-ERBB2 (KIFGSLAFL) TCR (clone E1), Jurkat Cell Line (TCRJ-CQ105)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The anti-ERBB2 (KIFGSLAFL) TCR (clone E1) Jurkat cell line is a stable cell line made from the anti-ERBB2 TCR lentivirus. The recombinant Jurkat T cell was designed to predict the MOA of ERBB2-TCR, measure the ERBB2-TCR specificity and screen target cells expressing ERBB2. And the product can be used to treat Breast cancer.

Specifications

Cell Background
The Jurkat cell line was established from the peripheral blood of human T lymphocyte cells. Jurkat cells are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors. Jurkat cells can produce interleukin 2, and are used in research involving the susceptibility of cancers to drugs and radiation.

Cell Type
T lymphocyte

Formulation
Containing ≥ 1 X 10*6 / vial frozen cells

Cell Purity
>95%

Cell Viability
>90%

Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Applications
• Screen for activators or inhibitors of CMV signaling in a cellular context
• Characterize the biological activity of CMV and its interactions with ligands
• Predict the MOA of the TCR design
• Screen and validate CMV-expressing target cells

Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.

Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Warnings
Avoid multiple freeze/thaw cycles

Research Use Only
Our recombinant Jurkat cell are for research use only, not for diagnostic or therapeutic use.

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.

Tumorgenicity
Positive (In vitro/vivo transformation assay)

Oncogenicity
Positive (In vitro soft agarose assay and life-time studies)

Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We
can provide guidance on the appropriate testing program upon your requirements.

TCR Design

Target
ERBB2

Introduction
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized

HLA
HLA-A2

Common Name
ERBB2

Target Species
Human

TCR Clone
E1

TCR-Host Animal
Human

Vector Name
pCDTCR1

Vector length
~ 8 kb

Vector Type
Lentiviral vector

Targeting Diseases
Breast cancer

Epitope
KIFGSLAFL

Format
Non-modified TCR

GeneID
2064

UniProt ID
P04626

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.