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TCR NKT15 "AAA" is derived from NKT15 TCR, which contains a triple alanine mutant in the 98-RDR-100 sequence (encoded by Dβ-N), which is highly solvent accessible and, as such, their mutation is not predicted to affect the conformation of the CDR3α loop. SPR-binding studies of this "AAA" mutant NKT15 TCR revealed its affinity for both human and mouse CD1d/α-GalCer was comparable to the wild-type NKT15, indicating these three exposed residues are not critical to the affinity of the interaction. In addition, this hybrid TCR inhibited staining of mouse NKT cells by mCD1d/α-GalCer tetramers at the same concentration as NKT12 and NKT15 TCRs.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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