Anti-GITR (X9X110) Negative Control CAR-Jurkat-NFAT-Luc (XS-0922-LX2299)

Online Inquiry Datasheet

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The Anti-GITR (X9X110) Negative Control CAR-Jurkat-NFAT-Luc cell line is a stable cell line derived from anti-GITR CAR lentivirus transduction using Jurkat-NFAT-luciferase cell as effector cell. This negative control consists of anti-GITR scFv linked to the CD28/CD8 transmembrane motif without any intracellular signaling domains.And the cell line can be used for the negative control of Anti-GITR (X9X110) CAR-Jurkat-NFAT-Luc.

Specifications

Species
Human

Host Cell Type
T lymphocyte

Overexpressed Gene
NFAT-Luciferase Reporter

Size
Containing ≥ 2 X 10*6 / vial lyophilized cells

Culture Condition
Suspension

Growth Pattern
Exponential growth phase

Formulation
Lyophilized cells

Cell Purity
>95%

Cell Viability
>90%

Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Shipping
Dry ice

Storage
Lyophilized cells should be stored in a liquid nitrogen tank (-150°C~-190°C).

Warnings
Avoid multiple freeze/thaw cycles

Research Use Only
Our recombinant Jurkat cells are for research use only, not for diagnostic or therapeutic use.

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.

Tumorgenicity
Positive

Oncogenicity
Positive (In vitro life-time studies)

Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

PROTOCOL
1. Thaw CAR-Jurkat cell samples quickly in a 37°C water bath until all visible ice has melted. Thaw time for a 1 ml sample in a cryovial is 2-3 minutes. Cryovials should be cool to the touch when removed from the water bath.
2. Dilute cell/medium mixture immediately with CAR-Jurkat cell culture medium. This can be performed in a single step. The dilution medium should be between 20-37°C. A dilution ratio of 1:10 (sample:medium) or greater is recommended.
3. Plate cells appropriately according to the experimental conditions of assays.
4. Culture the control lentivirus CAR-Jurkat cells or use immediately.
Note: Jurkat cells that are used immediately after thawing have the highest level of viability.

Application
1. Compound screening;
2. Antibody screening;
3. Co-stimulatory and activation domain comparison;
4. Checkpoint inhibitors screening;
5. Safety switches and regulators of CAR-Jurkat functions study;
6. Pre-clinical study in vivo models;
7. CAR-Jurkat signaling, tumor microenvironment;
8. Proof of concept studies for clinical trials;

CAR Design

Target
GITR

Target Species
Human

scFv Clone
X9X110

scFv Host Spcies
Human

CAR Construction
scFv-CD28/CD8TM

CAR Vector Name
pCDCAR1

CAR Vector length
~8kb

CAR Vector Type
Lentiviral vector

CAR Generation
First

Targeting Diseases
Autoimmune Diseases

GeneID
8784

Customize Your CAR Products

Cannot find the desired product? Don't worry, just try our online CAR and CAR cell customizing system, which offers full options to meet all unique needs, including but not limited to conventional or unconventional CAR constructs, as well as a variety of vectors and cells. The customization process can be completed with just a few simple clicks, please feel free to try it out.
CAR and CAR Cell Customizing System

Customer Reviews and Q&As

There are currently no customer reviews or questions for Anti-GITR (X9X110) Negative Control CAR-Jurkat-NFAT-Luc (XS-0922-LX2299). Click the button below to contact us or submit your feedback about this product.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Anti-GITR (X9X110) Negative Control CAR-Jurkat-NFAT-Luc (XS-0922-LX2299)

Specific Inquiry

Specifications

CAR Design

Customize Your CAR Products

Customer Reviews and Q&As

Related Products

CAR Vector Products

CAR Viral Particles

CAR Cell Products