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Anti-MAGE6 (F4)-CD147 CAR-MA (CARMA-W1165)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The vector of anti-MAGE6 chimeric antigen receptor (CAR) is constructed for the engineering of macrophage cells to target human MAGE6 and induce target cell phagocytosis. The macrophage cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-MAGE6 antibody (F4) linked to CD147 signaling domains. The vector product was designed for the treatment of NSCLC (MAGE-A3/6), metastatic melanoma (70% MAGE-A1-5).

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Details

  • Target
  • MAGE6
  • Cell Type
  • Macrophage
  • Targeting Diseases
  • NSCLC (MAGE-A3/6), metastatic melanoma (70% MAGE-A1-5)
  • Vector Name
  • pCDCAR1
  • Vector length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Size
  • 10 µg
  • Storage
  • Store at -20°C for long term.

CAR Components

  • Promoter
  • EF1a
  • scFv
  • F4
  • Hinge
  • IgG1
  • TM
  • CD147
  • ICD
  • CD147
  • Marker
  • GFP or N/A
  • Receptor Construction
  • scFv-hinge(IgG1)-TM(CD147)-ICD(CD147)
  • Discription of Signaling Cassetes
  • To breakdown the "physical barrier" of the tumour matrix, CD147 is used as the signaling domain. It's a membrane molecule that is essential for ECM remodelling via the expression of MMPs.

Target

  • Target
  • MAGE6
  • Target Species
  • Human
  • Clone
  • F4
  • Host
  • Mouse
  • Gene Name
  • MAGE family member A6
  • Introduction
  • This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. Alternative splicing results in multiple transcript variants.
  • Alternative Names
  • MAGEA6; MAGE family member A6; CT1.6; MAGE6; MAGE3B; MAGE-3b

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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