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Anti-MAGE6 (F4)-CD3ζ CAR THP1 (CARTHP-W1167)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The anti-MAGE6 chimeric antigen receptor (CAR) THP1 cell line is a stable cell line developed using anti-MAGE6 CAR lentivirus transfection. This transfected CAR lentiviral vector is constructed to express a scfv of anti-MAGE6 antibody (F4) linked to CD3ζ signaling domains.The anti-MAGE6 CAR engineered THP1 cells can be used to be differentiated into macrophage cells for in vitro or in vivo phagocytosis studies. This CAR-THP1 cell line product can be used for the researches of NSCLC (MAGE-A3/6), metastatic melanoma (70% MAGE-A1-5).

Specific Inquiry

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  • Marker:
  • Positive rate:
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Details

  • Target
  • MAGE6
  • Cell Type
  • THP1
  • Cell Background
  • THP-1 is a human leukemia monocytic cell line, which has been extensively used to study monocyte/macrophage functions, mechanisms, signaling pathways, and nutrient and drug transport. This cell line has become a common model to estimate modulation of monocyte and macrophage activities. CAR engineered THP1 cells can be differentiated into macrophages using PMA (Phorbol 12-myristate 13-acetate). Once differentiated (M0 macrophages), they were incubated with IL-4 and IL-13 in order to obtain M2 polarized macrophages or with IFN-gamma and LPS for classical macrophage activation (M1).
  • Targeting Diseases
  • NSCLC (MAGE-A3/6), metastatic melanoma (70% MAGE-A1-5)
  • CAR Expression
  • Positive
  • CAR Positive Rate
  • >95% for stable cell pool (please submit an inquiry if single clonal cell line is needed.)
  • Cell Viability
  • >90%
  • Mycoplasma Testing
  • Negative
  • Handling Notes
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
    Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
    Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
  • Safety Consideration
  • Biosafety Level 1
  • Restriction
  • Research use only
  • Size
  • 2 million cells*2 vials
  • Storage
  • Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C) for long term.

CAR Components

  • Promoter
  • EF1a
  • scFv
  • F4
  • Hinge
  • CD8
  • TM
  • CD8
  • ICD
  • CD3ζ
  • Marker
  • GFP or N/A
  • Receptor Construction
  • scFv-hinge(CD8)-TM(CD8)-ICD(CD3ζ)
  • Discription of Signaling Cassetes
  • CD3ζ:
    CD3ζ is used as the CAR intracellular signaling domain. CD3ζ has homology to the Fc common γ-chain, FcεRI-γ, a canonical signaling molecule for antibody_x005f_x005f_x005F_x005f_x005F_x0002_dependent cellular phagocytosis (ADCP) in macrophages. CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Target

  • Target
  • MAGE6
  • Target Species
  • Human
  • Clone
  • F4
  • Host
  • Mouse
  • Gene Name
  • MAGE family member A6
  • Introduction
  • This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. Alternative splicing results in multiple transcript variants.
  • Alternative Names
  • MAGEA6; MAGE family member A6; CT1.6; MAGE6; MAGE3B; MAGE-3b

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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