Anti-Myelin Basic Protein T Cells, Donor 3 (ICZP127)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The BC3 cell line was isolated from a normal 56 year old Caucasian man. In vitro stimulation of peripheral blood mononuclear cells (PBMC) with a peptide of MBP was used to select for peptide reactive T cells. The peptide used (ENPVVHFFKNIVTPRTP) is believed to be the immunodominant epitope. The cell line is likely oligoclonal and recognizes the peptide presented by HLA-DRβ1*1501 and may also recognize peptide presented by HLA-DRβ1*14, though this has not yet been confirmed. The cells also recognize the peptide FFKNIVTPRTPPPSQGK, thus the minimal epitope for this line is FFKNIVTPRTP. The cells secrete interferon gamma, IL-13 and TNF alpha upon stimulation. They are CD4+ and, not surprisingly, FoxP3 negative.

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Donor Information

Product Name
Anti-Myelin Basic Protein T Cells, Donor 3

Cat. No.
ICZP127

Gender
Male

Donor Age
56

Donor Health
Healthy

HLA-B
HLA-B: 14,27:05

HLA-DRβ1
HLA-DRβ 1: 15:01,14

Blood Type
Unknown

Ethnicity
Caucasian

Additional Information

Cell Purity
>95%

Cell Viability
>90%

Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

Preparation
The BC3 cell line was isolated from a normal 56 year old Caucasian man. In vitro stimulation of peripheral blood mononuclear cells (PBMC) with a peptide of MBP was used to select for peptide reactive T cells. The peptide used (ENPVVHFFKNIVTPRTP) is believed to be the immunodominant epitope. The cell line is likely oligoclonal and recognizes the peptide presented by HLA-DRβ1*1501 and may also recognize peptide presented by HLA-DRβ1*14, though this has not yet been confirmed. The cells also recognize the peptide FFKNIVTPRTPPPSQGK, thus the minimal epitope for this line is FFKNIVTPRTP. The cells secrete interferon gamma, IL-13 and TNF alpha upon stimulation. They are CD4+ and, not surprisingly, FoxP3 negative.

Application
Flow Cytometry; MAT assays

Research Use Only
Our recombinant Jurkat cell are for research use only, not for diagnostic or therapeutic use.

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.

Storage
vapour phase nitrogen

Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Warnings
Avoid multiple freeze/thaw cycles

Size
1-5M cells/vial

Keywords
ICZP127; Anti-Myelin Basic Protein T Cells, Donor 3; Anti-Myelin Basic Protein T Cells

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.