PBMC Products

Characteristics of Human PBMC

Peripheral blood mononuclear cells (PBMC) are a subpopulation of leukocytes, which serve as the dominant cells of the body's immunity system that generate selective responses to the immune system against diseases. The position of PBMCs is very important as they serve as a defense line against infection and disease. PBMC cells are usually isolated from whole blood samples. PBMCs consist of a variety of specialized cell populations, such as monocytes, lymphocytes, macrophages, and dendritic cells, these different cells play specific functions and are used in medical fields.

Fig.1 PBMC subsets identified by flow cytometry analysis.¹

PBMC Isolation

Generally, there are two main PBMC isolation methods, ficoll-density gradient centrifugation and red blood cell lysis. Ficoll-density gradient centrifugation is a cheap and simple method for PBMC isolation. Ficoll is a type of hydrophilic polysaccharide used to separate the blood layers. Ficoll-density gradient centrifugation separates the blood into the top layer of plasma, followed by the bottom layer of PBMCs, then a subset of polymorphonuclear cells (such as eosinophils and neutrophils), and finally the bottom red blood cells layer.

Fig.2 Experimental protocol for isolating human peripheral blood mononuclear cells from whole blood.²

The Applications of PBMCs

Compared with isolated cells, PBMCs as an integral cell population that plays a critical role in the constructing and maintaining effective immune response. Based on the feature, several researchers use PBMC to exploit the immune responses of drug candidates. There are multiple research areas applications for PBMCs including:

• Drug toxicity assessment
• Cell therapy
• Disease modeling
• Biomarker identification
• Cancer immunology study
• Infectious diseases study
• Vaccine development
• Personalized medicine development

PBMCs Cells at Creative Biolabs

In order to better help global customers achieveing the success of the project, Creative Biolabs provides a variety of isolated Peripheral Blood Mononuclear Cells (PBMCs) from whole blood obtained from IRB-consented donors. Our ready-for-use PBMC products are offered with strict and standardized QC data. Meanwhile, we are committed to providing customized cell products according to global customers' variable needs. Our PBMC cell products with the following features:

• Our specimens are followed by IRB approval.
• Products are available in custom formats with special donor requirements.
• Strict quality control ensures consistent, reliable cell batches.
• Standard collection protocol of PBMC cell products
• High quality, purity, and viability for use

Here are some hot cell products you can browse to find a suitable product for your project. For more defined requirements, please feel free to contact us or send us an inquiry.

References

1. Obasanmi, Gideon, et al. "Peripheral blood mononuclear cells from patients with type 1 diabetes and diabetic retinopathy produce higher levels of IL-17A, IL-10 and IL-6 and lower levels of IFN-γ—A pilot study." Cells 12.3 (2023): 467.
2. Mendez-David, Indira, et al. "A method for biomarker measurements in peripheral blood mononuclear cells isolated from anxious and depressed mice: β-arrestin 1 protein levels in depression and treatment." Frontiers in Pharmacology 4 (2013): 124.