Human Magnetic Luminex Mutiplex Cytokine/Chemokine Array Kit-41 Plex C (CAR-K-LX-022)

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Product Name
Human Magnetic Luminex Mutiplex Cytokine/Chemokine Array Kit-41 Plex C

Cat. No.
CAR-K-LX-022

Product Summary
Human Cytokine / Chemokine panel enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.

Components
96-Well Plates, Assay Buffer, Wash Buffer, Detection Antibodies, Bead Diluent, Beads, Standard

Precision
Intra-assay: <15
Inter-assay: <15

Applications
Simultaneously analyze multiple cytokine and chemokine and other biomarkers with Bead-Based Multiplex Assays using the Luminex technology.

Product format/Physical form
96-well microplate

Assay Type
Multiplex

Cross-reactivity
No significant cross-reactivity was observed among analytes in this panel.

Species Reactivity
Human

Sample Type (General)
Human serum, plasma and cell culture samples.

Shipping Condition
The product is recommended to be shipped on dry ice.Upon receipt, store immediately at recommended temperature below.

Storage Conditions
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

Plex size
41

Dtection Coverage
sCD40L, EGF, Eotaxin/CCL11, FGF-2, Flt-3 ligand, Fractalkine, G-CSF, GM-CSF, GRO, IFN-α2, IFN-γ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IP-10, MCP-1, MCP-3, MDC (CCL22), MIP-1α, MIP-1β, PDGF-AA, PDGF-AB/BB, RANTES, TGF-α, TNF-α, TNF-β, VEGF

Performance Validation
Our Luminex Assays undergo our most extensive validation testing. All Luminex Assays are validated for use with serum and plasma samples and tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types.

Detection method
Fluorescent

Product Features
Availability: able to detect analytes across a large-scale of concentrations reliably.
High throughput multiplex analysis.
Efficiency in terms of time and cost for multiple targets.

Principle of the Assay
To identify specific cytokines involved in any inflammatory or immune response, it might be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing.

Research Category
Inflammation & Immunology

Application Notes
This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended.
This assay requires 25 µl of neat plasma or serum, or 25 μL cell culture supernatant per well.
This kit may be used for the analysis of all or any combination of the cytokines and chemokines in serum, plasma or tissue/cell lysate and culture supernatant samples with the exception of RANTES, PDGF-AA and PDGF-AB/BB.
RANTES, PDGF-AA and PDGF-AB/BB cannot be plexed with other cytokines in this panel due to a required 1:100 dilution of plasma/serum samples.

Accuracy
87% - 107%

Panel Type
MAGNETIC Cytokines/Chemokines/Other Markers

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.