Anti-EGFR (7B3) h(CD28-CD3ζ) CAR, pCDCAR1 vector

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Details

Target
EGFR

Targeting Cell Type
T cell

Targeting Diseases
Melanoma

Generation
Second

Vector Name
pCDCAR1

Vector Length
~8kb

Vector Type
Lentiviral vector

Receptor Construction
scFv-CD28-CD3ζ

Discription of Signaling Cassetes
CD28
CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide costimulatory signals required for T cell activation and survival. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins which are expressed on antigen-presenting cells (APC). CD28 modulates the primary TCR/CD3ζ signal in a different fashion than the late costimulatory elements OX40 and 4-1BB. CD28 enhances the expression of downstream regulators that impact on T-cell proliferation, death, differentiation, and effector functions. CAR+ T cells containing the CD28 endodomain showed strikingly enhanced sustained T cell activation, growth, survival. And CD28 results in a brightly expressed, stable receptor as the transmembrane domain. Including CD28 costimulatory domains in CARs led to enhanced anti-malignancy efficacy.
CD3ζ
CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Target

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Published Data

CAR scFv data FCM

Fig.1 FACS analysis demonstrated the binding of scFv-M27-Fc, scFv-806-Fc, and scFv-C225-Fc to the indicated target cells.

CAR Construction :

Fig.1 FACS analysis demonstrated the binding of scFv-M27-Fc, scFv-806-Fc, and scFv-C225-Fc to the indicated target cells.

Fluorescence was assessed by using a BD FACSCelesta flow cytometer.

Jiang, H., Gao, H., Kong, J., Song, B., Wang, P., Shi, B., ... & Li, Z. (2018). Selective Targeting of Glioblastoma with EGFRvIII/EGFR Bitargeted Chimeric Antigen Receptor T CellEGFR/EGFRvIII Bitargeted CAR T Cells for Cancer Treatment. Cancer Immunology Research, 6(11), 1314-1326.

Complete CAR data FCM

Fig.2 Characterization of EGFR/EGFRvIII bitargeted CAR T cells.

CAR Construction : 7B3 scFv-28ζ

Fig.2 Characterization of EGFR/EGFRvIII bitargeted CAR T cells.

Expression of EGFR-specific CARs on the lentivirus-transduced human T cells was analyzed using flow cytometry.

Complete CAR data Cyt

Fig.3 In vitro cytotoxic activities of EGFR/EGFRvIII-targeted CAR T cells.

CAR Construction : 7B3 scFv-28ζ

Fig.3 In vitro cytotoxic activities of EGFR/EGFRvIII-targeted CAR T cells.

Primary human T cells transduced with the indicated lentiviral vectors were incubated with the untransfected and transfected glioblastoma cells at
the various E:T ratios for 18 hours.

Complete CAR data FuncS

Fig.4 In vivo antitumor activities of M27-28BBZ CAR T cells on established subcutaneous EGFR- or EGFRvIII-overexpressing glioblastoma xenografts.

CAR Construction : 7B3 scFv-28ζ

Fig.4 In vivo antitumor activities of M27-28BBZ CAR T cells on established subcutaneous EGFR- or EGFRvIII-overexpressing glioblastoma xenografts.

Data are presented as the mean tumor volume ± SEM.

Complete CAR data FuncS

Fig.5 The persistence of human T cells from mice treated with the indicated genetically modified T cells in orthotopic glioblastoma xenografts models.

CAR Construction : 7B3 scFv-28ζ

Fig.5 The persistence of human T cells from mice treated with the indicated genetically modified T cells in orthotopic glioblastoma xenografts models.

The representative immunostaining images of CD3þ T-cell infiltration in tumor-bearing mice brains.

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.