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pCDCAR1 EGFR h(28ζ) (CAR-ZP8190)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The vector of anti-EGFR chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target Human EGFR. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-EGFR antibody linked to CD28 and CD3ζ signaling domains. And the vector product was designed for the treatment of Melanoma.

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Details

  • Target
  • EGFR
  • Targeting Cell Type
  • T cell
  • Targeting Diseases
  • Melanoma
  • Generation
  • Second
  • Vector Name
  • pCDCAR1
  • Vector Length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Receptor Construction
  • scFv-CD28-CD3ζ
  • Discription of Signaling Cassetes
  • CD28
    CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide costimulatory signals required for T cell activation and survival. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins which are expressed on antigen-presenting cells (APC). CD28 modulates the primary TCR/CD3ζ signal in a different fashion than the late costimulatory elements OX40 and 4-1BB. CD28 enhances the expression of downstream regulators that impact on T-cell proliferation, death, differentiation, and effector functions. CAR+ T cells containing the CD28 endodomain showed strikingly enhanced sustained T cell activation, growth, survival. And CD28 results in a brightly expressed, stable receptor as the transmembrane domain. Including CD28 costimulatory domains in CARs led to enhanced anti-malignancy efficacy.
    CD3ζ
    CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Target

  • Clone
  • 151
  • Host
  • Mouse
  • Target Species
  • Human
  • Gene Name
  • Epidermal Growth Factor Receptor
  • Synonyms
  • EGFR;EGFR; Epidermal Growth Factor Receptor; Epidermal Growth Factor Receptor; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; ERBB1; ERBB; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian);

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  • Published Data
CAR scFv data ELISA

Fig.1 MM-151 blocks signal amplification by the high-affinity EGFR ligand EGF (8 nmol/L) more effectively than cetuximab.

CAR Construction : Latest CAR Construction

Fig.1 MM-151 blocks signal amplification by the high-affinity EGFR ligand EGF (8 nmol/L) more effectively than cetuximab.

MM-151 elicits superior ligand antagonism versus high-affinity ligands.

Kearns, J. D., Bukhalid, R., Sevecka, M., Tan, G., Gerami-Moayed, N., Werner, S. L., ... & Wolf, B. B. (2015). Enhanced Targeting of the EGFR Network with MM-151, an Oligoclonal Anti-EGFR Antibody TherapeuticEnhanced Inhibition of EGFR Signal Amplification by MM-151. Molecular Cancer Therapeutics, 14(7), 1625-1636.
CAR scFv data FuncS

Fig.2 MM-151 is superior to cetuximab at blocking tumor growth of a high-affinity EGFR ligand–expressing murine xenograft model.

CAR Construction : Latest CAR Construction

Fig.2 MM-151 is superior to cetuximab at blocking tumor growth of a high-affinity EGFR ligand–expressing murine xenograft model.

Xenograft tumors of H322M-HB-EGF cells were established in immunecompromised mice and treated with PBS, indicated doses of cetuximab, or indicated cetuximab-equivalent doses of 25E-Trio, and tumor volumes were measured by calipers.

Kearns, J. D., Bukhalid, R., Sevecka, M., Tan, G., Gerami-Moayed, N., Werner, S. L., ... & Wolf, B. B. (2015). Enhanced Targeting of the EGFR Network with MM-151, an Oligoclonal Anti-EGFR Antibody TherapeuticEnhanced Inhibition of EGFR Signal Amplification by MM-151. Molecular Cancer Therapeutics, 14(7), 1625-1636.

More Published Data More Published Data

Customer Reviews and Q&As

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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