CHRNB4 Membrane Protein Introduction

Introduction of CHRNB4

Neuronal acetylcholine receptor subunit beta-4 is a protein encoded by the CHRNB4 gene with a molecule weight of 56 kDa. The CHRNB4 membrane protein has a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. This CHRNB4 membrane protein can be combined to alpha-2, alpha-3 or alpha-4 to give rise to functional receptors, like neuronal nicotinic acetylcholine receptor complex, alpha3-alpha6-beta4.

Basic Information of CHRNB4
Protein Name	Neuronal acetylcholine receptor subunit beta-4
Gene Name	CHRNB4
Aliases
Organism	Homo sapiens (Human)
UniProt ID	P30926
Transmembrane Times	4
Length (aa)	498
Sequence	MRRAPSLVLFFLVALCGRGNCRVANAEEKLMDDLLNKTRYNNLIRPATSSSQLISIKLQLSLAQLISVNEREQIMTTNVWLKQEWTDYRLTWNSSRYEGVNILRIPAKRIWLPDIVLYNNADGTYEVSVYTNLIVRSNGSVLWLPPAIYKSACKIEVKYFPFDQQNCTLKFRSWTYDHTEIDMVLMTPTASMDDFTPSGEWDIVALPGRRTVNPQDPSYVDVTYDFIIKRKPLFYTINLIIPCVLTTLLAILVFYLPSDCGEKMTLCISVLLALTFFLLLISKIVPPTSLDVPLIGKYLMFTMVLVTFSIVTSVCVLNVHHRSPSTHTMAPWVKRCFLHKLPTFLFMKRPGPDSSPARAFPPSKSCVTKPEATATSTSPSNFYGNSMYFVNPASAASKSPAGSTPVAIPRDFWLRSSGRFRQDVQEALEGVSFIAQHMKNDDEDQSVVEDWKYVAMVVDRLFLWVFMFVCVLGTVGLFLPPLFQTHAASEGPYAAQRD

Function of CHRNB4 Membrane Protein

Neuronal acetylcholine receptor subunit beta-4 is ubiquitously present on airway epithelial cells and forms heteromeric nAchRs to modulate receptor affinity for nicotine. Genome-wide association studies have revealed that chromosome region 15q25, which harbors the CHRNB4 gene, is associated with an increased risk for nicotine dependence in European, American and Asian populations. Beyond that, CHRNB4 is strongly associated with genetic lung cancer susceptibility variants and a functional impact on tumorigenic potential. Two contradicting findings report that CHRNB4 plays a potential tumor suppressor or oncogenic role in lung cancer. Therefore, it is conceivable that the role of CHRNB4 in lung cancer is diverse, depending on the cellular subtypes or tumor stages.

Fig.1 Evidence supporting the repurposing of bupropion for smoking cessation. This figure shows the 10 most supportive paths (out of 365 total) for treating nicotine dependence with bupropion, as available in this prediction’s Neo4j Browser guide. (Himmelstein, 2017)

Application of CHRNB4 Membrane Protein in Literature

Yoo S.S., et al. Unmethylation of the CHRNB4 gene is an unfavorable prognostic factor in non-small cell lung cancer. Cell reports. 2014, 86(1):85-90. PubMed ID: 25172267

This article finds that epigenetic regulation of CHRNB4 may affect tumor progression and survival in patients with NSCLC.
Zhao Z., et al. Exon sequencing identifies a novel CHRNA3-CHRNA5-CHRNB4 variant that increases the risk for chronic obstructive pulmonary disease. Respirology. 2015, 20(5):790-8. PubMed ID: 25891420

This article suggests that two previously reported variants Tyr215Tyr and Asp398Asn of CHRNA3-CHRNA5-CHRNB4 and a novel exon variant Ser140Gly are significantly related to COPD risk in Chinese. These variants may be genetic biomarkers for predicting COPD risk in Chinese.
Gallego X., et al. Alternative CHRNB4 3'-UTRs mediate the allelic effects of SNP rs1948 on gene expression. PLoS One. 2013, 8(5):e63699. PubMed ID: 23691088

This article suggests the importance of SNP rs1948 on the regulation of CHRNB4 expression and provides the first evidence of CHRNB4 down-regulation by miR-138.
Chen L.S. and Bierut L.J. Genomics and personalized medicine: CHRNA5-CHRNA3-CHRNB4 and smoking cessation treatment. Journal of Food and Drug Analysis. 2013, 21(4):S87-S90. PubMed ID: 25214750

This article reveals that variants in the CHRNA5-CHRNA3-CHRNB4 region identify individuals at increased risk of cessation failure, and this increased risk can be ameliorated by cessation pharmacotherapy.
Scherf D.B., et al. Epigenetic screen identifies genotype-specific promoter DNA methylation and oncogenic potential of CHRNB4. Oncogene. 2013, 32(28):3329-38. PubMed ID: 22945651

This article suggests that CHRNB4 is strongly associated with genetic lung cancer susceptibility variants and a functional impact on tumorigenic potential.

CHRNB4 Preparation Options

Membrane protein studies have advanced significantly over the past few years. Based on our versatile Magic™ membrane protein production platform, we could offer a series of membrane protein preparation services for worldwide customers in reconstitution forms as well as multiple active formats. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-CHRNB4 antibody development services.

During the past years, Creative Biolabs has successfully generated many functional membrane proteins for our global customers. We are happy to accelerate the development of our clients’ programs with our one-stop, custom-oriented service. For more detailed information, please feel free to contact us.

Reference

Himmelstein D S, et al. (2017). Systematic integration of biomedical knowledge prioritizes drugs for repurposing. Elife.6.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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