Creative Biolabs offers high-quality serum and plasma products. Our blood products are collected under FDA-approved protocols in the United States. Featured complement activity-preserved products are available for biocompatibility experiments including drug development, biomaterials testing, lymphocytotoxicity, and hemolytic procedure.
We also provide a range of serum and plasma products that depleted the special protein. We are dedicated to offering high-quality products to help your project to succeed.
Complement serums and plasma are vital components in the study of immunology and biological research. These biological fluids, derived from various organisms, play crucial roles in the immune response, particularly in the complement system.
A fraction of blood obtained after coagulation, complement serum contains active proteins that have yet to be consumed during clot formation. It's widely used in research applications, especially in complement activity assays.
Plasma, unlike serum, is extracted from blood before coagulation, containing fibrinogen and other clotting factors. It is a critical resource for studies requiring both complement proteins and clotting factors.
These materials are sourced from various species, such as human, mouse, and rat, and are indispensable in investigating the immune responses, conducting hemolysis tests, and performing antibody screening assays.
Types of Complement Serums and Plasma
Different complement serums and plasma are available for specific research needs, depending on the organism from which they are derived and their intended application.
Human Complement Serum and Plasma
Applications: Are widely used for immune response modeling, disease progression studies, and vaccine efficacy testing.
Research Focus: Particularly useful in antibody screening assays.
Mouse Complement Serum and Plasma
Applications: Are frequently employed in preclinical studies, genetic studies and immunological experiments.
Research Focus: Popular in studies involving hemolysis assays and mouse model testing for autoimmune diseases and inflammation.
Rat Complement Serum and Plasma
Applications: Are commonly used in pharmacological research and toxicology.
Research Focus: Used in in vitro and in vivo studies to evaluate complement-mediated cytotoxicity and drug effects on immune system modulation.
Non-human Primate (NHP) Complement Serum and Plasma
Applications: Are considered the closest to human complement products and are used extensively in translational research.
Research Focus: Ideal for research focused on understanding human-like immune responses in preclinical studies, particularly in xenotransplantation and immune therapy development.
Product Collection Specifications
Need a custom product? Let us know how we can customize any product to meet your specific project needs.
We conduct an in-depth comparison of Human vs. Animal Complement Serum, examining their functional characteristics, research applications, and species compatibility.
Attribute | Human | Mouse | Rat | Rabbit | Guinea Pig | Bovine |
---|---|---|---|---|---|---|
Function | Used in human immunology research, autoimmune disease studies | Used in genetic and immunological studies for mouse models | Used in toxicology and infection models | Highly reactive complement, used in lysis assays | Considered more potent in hemolytic assays | Used in studies where cross-species complement is required |
Pathway Activation | Activates classical, alternative, and lectin pathways, critical for human immunity | All pathways active, closely mimicking human complement activation | Strong activation of classical and alternative pathways, often used in inflammation studies | Classical pathway dominates, highly efficient in lysis studies | Excellent for alternative pathway research due to sensitivity | Strong classical pathway, sometimes used as a substitute for human complement |
Hemolytic Activity | High specificity to human erythrocytes | Moderate hemolytic activity, specific to murine erythrocytes | Moderate hemolytic activity, particularly in inflammatory contexts | Very high hemolytic activity, ideal for complement fixation assays | Exceptional hemolytic activity, used in classical pathway assays | Lower hemolytic activity compared to human and rabbit sera |
Research Applications | Autoimmune disease models, infectious disease research, vaccine development, immune regulation | Used in transgenic mouse models, antibody development, immune response modulation | Toxicology, infection models, inflammation studies | Classical pathway activation for complement fixation, hemolysis, and antibody development | Strong alternative pathway studies, excellent for testing immune system compatibility | Veterinary research, xenotransplantation models, antibody production |
Species Compatibility | Specifically reacts with human cells and proteins, high species specificity | Strong species-specific reactions with murine cells, but limited cross-reactivity with other species | Works well within rat models but limited in cross-species applications | Useful in complement lysis studies, particularly for rabbit-specific assays | Often used as a model for alternative pathway reactions in other species | Cross-reactivity with other mammals, particularly in xenotransplantation |
Availability | Commercially available in purified and native forms | Readily available | Readily available | Readily available | Readily available | Readily available |
Research Objectives
If so, species-specific serum containing active complement proteins and antibodies may be required.
Many researchers prefer fetal bovine serum (FBS) due to its rich content of growth factors, but human or specialized sera may be necessary for certain models.
You'll need serum or plasma free from coagulation factors that could interfere with your assays.
Species Source
You may refer to the table above to make your selection.
Purity and Processing
You'll need to consider how the biological material is processed to ensure that it matches your experimental needs. Important factors include:
Experimental Conditions
Matching serum or plasma to your experimental conditions is essential for reproducibility and validity. Consider factors such as:
Creative Biolabs provides a comprehensive range of complement sera and plasma products, tailored to meet the advanced demands of scientific and pharmaceutical research. Our technical guide provides detailed protocols and best practices for using complement sera and plasma in scientific assays.
Key Applications
Complement sera are commonly used in both in vitro and in vivo assays, including hemolytic complement assays, complement-mediated cytotoxicity (CMC), immunoassays for complement activation, complement pathway activation analysis.
Classical Pathway Hemolytic Assay (CH50 Assay)
The CH50 assay is used to measure the functional activity of the classical pathway by evaluating the lysis of antibody-coated sheep erythrocytes.
Alternative Pathway Assay (AP50 Assay)
The AP50 assay measures the activity of the alternative complement pathway, which can be activated in the absence of antibodies and is critical for pathogen defense.
Lectin Pathway Assay
The Lectin Pathway is activated by carbohydrate recognition molecules, such as mannose-binding lectin (MBL). This assay is useful in studying innate immune responses and carbohydrate-lectin interactions.
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Creative Biolabs offers a range of high-quality products for complement activity
and function assays. View our product list to find your interested products.
Complement Function Analysis Protocols
We briefly summarize the complement-related assay protocols. Find complement-related assay protocols by clicking the following items.
Tips for Experimental Success
We conduct an in-depth comparison of Human vs. Animal Complement Serum, examining their functional characteristics, research applications, and species compatibility.
Complement proteins are heat-labile, meaning they are highly sensitive to temperature fluctuations. Proper storage is critical to preserving complement activity. Short-term storage: Complement serum and plasma should be stored at -20°C or lower for up to one month. Long-term storage: For extended periods (over a month), storage at -80°C is advised to ensure optimal complement activity. Thawing should always be performed on ice or at 4°C to prevent denaturation. Repeated freeze-thaw cycles should be avoided, as they can degrade complement components and reduce functional activity.
When working with complement serum or plasma, several best practices must be followed to ensure experimental validity:
Complement-depleted serum or plasma refers to biological samples where specific complement proteins (such as C3, C4, or C5) have been removed or inactivated. These depleted products are critical for investigating individual complement pathways and validating complement-related therapies. By using depleted serum or plasma, scientists can evaluate the therapeutic potential of complement inhibitors or enhancers in isolation from other immune factors.
Complement-depleted serum or plasma refers to biological samples where specific complement proteins (such as C3, C4, or C5) have been removed or inactivated. These depleted products are critical for investigating individual complement pathways and validating complement-related therapies. By using depleted serum or plasma, scientists can evaluate the therapeutic potential of complement inhibitors or enhancers in isolation from other immune factors.
At Creative Biolabs, we offer customized complement preparations to meet the unique needs of your research. These services include:
Please contact our team to discuss your specific requirements, and we will work to deliver high-quality, scientifically validated products to support your research.