In recent years, great progress has been made in complement test to better define disease’ severity and to explore the development of complement-targeted therapies. Creative Biolabs has extensive experience in complement test, providing customers with a full range of test services, including complement function/activity test, autoantibody test, and genetic test, which address the significantly unmet need for accurate and timely diagnosis and monitoring of complement-related disease.
Complement test is a blood test that measures the activity of a group of proteins in the bloodstream. These proteins make up the complement system, which is one part of the immune system. The complement system helps antibodies fight off infections and destroy substances that are foreign to the body. However, when the following conditions occur in the complement system: (1) abnormal function of complement components and activation products, (2) production of autoantibody to complement protein, (3) mutations in the complement gene, it may lead to autoimmune diseases and other inflammatory reactions. Complement tests contribute to determining whether deficiencies or abnormalities in the complement system are occurring or contributing to the patient’s disease or condition.
The following tests are performed at Creative Biolabs:
Complement Test Can Be Used To:
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With abundant experience in complement test, Creative Biolabs can bring consistent and efficient test services and results that can enhance your research. For more information, please feel free to contact us.
Fig.1 C Functional evaluation of complement activation through hemolytic assay techniques.1
To investigate complement activation initiated by antibodies under therapeutic hypothermia conditions, researchers performed functional hemolytic assays at these temperatures. Specifically, they tested antibody-sensitized sheep erythrocytes incubated in normal human serum and assessed hemolysis. In this CH50-type assay, the degree of hemolysis varied notably with temperature changes. The findings revealed that antibody-induced complement activation was elevated at clinical hypothermia temperatures compared to normal body temperatures. This suggests that such activation during reperfusion at lower temperatures might enhance complement-driven tissue damage.
Reference
A: Hemolysis assays have traditionally been used to assess the functional activity of the complement system. They provide insight into the integrity of the entire cascade response. These tests are particularly useful when investigating suspected complement defects. Hemolysis tests are performed in test tubes or agarose plates. In the experiment, serial dilutions of the sample to be analyzed are incubated with antibody-sensitized sheep erythrocytes at a defined temperature.
A: Complement tests may include functional analysis, specifically encompassing total complement activity of the classical, lectin and alternative pathways, activity of individual components, C1 inhibitor activity, etc., as well as immunochemical analysis of individual components, analysis of complement activation products, determination of complement binding autoantibodiesd etc.
A: (a) In vitro experiments with human serum and blood. We can easily study the activation mechanism and intervention of complement activation in vitro with human serum. (b) Animal experiments. We can apply genetically defective or knockout animals, such as those with genetic defects in individual complement components. (c) Testing animal complement. Hemolysis assays can be designed for any species.
A: The time can vary depending upon the complexity of the tests being conducted, but overall you can usually expect results within 2 to 3 weeks.
A: We can work with a variety of samples, including serum, plasma, whole blood, and tissue samples. Please prepare them according to our guidelines to ensure the best results.
A: The sample storage and transport methods would depend on the type of sample involved. However, we generally advise for samples to be stored frozen and transported on ice to ensure their viability.
A: We have a team of experts on hand to assist you in understanding your results and their implications for your research.