Cell-based Complement Activity Assay

Creative Biolabs offers high-quality cell-based complement activity assays to help your research and drug development projects. Our services represent a comprehensive solution for researchers seeking to understand and manipulate the complex dynamics of the complement system.

Overview Featured Services Applications Key Features Related Services

Overview

The complement system is an integral part of the immunity response and plays a vital role in defense against pathogens and immune regulation. Cell-based complement activity assays enable the direct assessment of complement activation and its consequences on target cells, mimicking in vivo conditions more closely than traditional biochemical assays. Therefore, cell-based complement activity assays have gradually emerged as a powerful tool to study the complex interactions between complement components and their target cells, providing insights into physiological and pathological processes.

Fig.1 A schematic representation of complement activation Fig. 1 Complement activation pathways.¹

Featured Services

At Creative Biolabs, we provide seamless end-to-end cell-based complement activity assay services from assay design to data analysis. Our experienced scientists rely on advanced methods and well-established procedures to ensure your complement activity assay project runs smoothly.

Indirect Immunofluorescence Assay (IFA Assay)

The cell-based complement activity assay, specifically the IFA assay, is a powerful and versatile technique utilized in immunology and biomedical research to evaluate the functional activity of the complement system. Following are simple protocols of the IFA assay.

Fig.2 The general flow of IFA assay. (Creative Biolabs Original)

Cell-Enzyme-Linked Immunosorbent (ELISA) Assay

Cell-ELISA is a sophisticated technique employed in biomedical research and diagnostics to evaluate the activation and functionality of the complement system within a cellular context. This assay marries the principles of traditional ELISA with the unique ability to monitor complement-mediated events directly on or near cell surfaces, providing a more physiologically relevant assessment compared to assays performed in solution.

Fig.3 The general flow of Cell-ELISA assay. (Creative Biolabs Original)

Applications

Disease Pathogenesis

Illuminated the role of complement in autoimmune diseases, infectious diseases, and inflammatory conditions.

Therapeutic Development

Evaluating the efficacy of complement inhibitors or activators in modulating cellular responses, support the discovery and development of novel therapeutic strategies.

Drug Safety Testing

Assessing the potential for drugs to activate or inhibit the complement system is crucial for predicting adverse immune reactions and optimizing drug safety profiles.

Why Choose Us?

Expertise & Experience

Our team of highly skilled scientists boasts extensive experience in immunology and cell-based assays, ensuring the highest quality results.

Customizable Solutions

We tailor our protocols to your specific research needs, adjusting antibody concentrations, and incubation times, or even incorporating additional controls as required.

Rigorous Quality Control

Every step is rigorously validated using industry-standard practices, ensuring reproducibility and reliability of your data.

Comprehensive Reporting

Detailed experimental reports, including raw data, analysis, and interpretation, are provided to empower your research insights.

Related Services

Reference

Ng, Nicole, and Charles A. Powell. "Targeting the complement cascade in the pathophysiology of COVID-19 disease." Journal of Clinical Medicine 10.10 (2021): 2188. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only.