Complement Multiplex Assay Services

Overview of Complement System

The complement system is composed of dozens of proteins and is an integral part of the immune response. This system includes three activation pathways that cross each other. In general, the complement system forms a membrane attack complex on the target microorganism through a complex cascade reaction, leading to the lysis of the target microorganism.

Fig.1 Schematic illustration of the complement cascade.^1,3

Related Diseases

The complement system is reported to be related to the occurrence of some diseases, such as asthma. In addition, this system also plays an important role in some autoimmune diseases, a typical example is multiple sclerosis. It is worth noting that increasing evidence shows that the complement system may be closely linked to spinal cord injury and neurological diseases. Therefore, it is necessary to detect multiple complement factors in the process of disease diagnosis and treatment.

Multiplex Assay Services

Key Features

• Higher accuracy

Excellent linearity and recovery rate.

• Smaller sample size

Few samples, more data, wide dynamic range detection accuracy.

• Diversified sample types

Suitable for various sample types, such as plasma and tissue lysate.

• High cost-effectiveness

The more indicators, the lower the cost of a single indicator.

• Batch-to-batch consistency

Kits for analytical services meet strict manufacturing standards ensuring batch-to-batch reproducibility.

• Customizable

A variety of complement proteins and factors can be matched based on your specific needs.

• High specificity

Our complement multiplex assay services reduce nonspecific binding and cross-reactivity to ensure high specificity.

Published Data

Fig.2 Novel multiplex suspension array for detecting complement activation in OSCC cells.^2,3

Oral squamous cell carcinoma (OSCC) represents a common neoplasm in the oral cavity, underscoring the necessity for early detection and prognostic strategies. This research focused on evaluating complement system activation in individuals with OSCC, suggesting its utility as a potential biomarker. An innovative complement activation array using targeted antibodies against complement epitopes C3a, C5a, and sC5b-9 was developed within a suspension bead array format and employing serum from both healthy individuals and OSCC patients, the array explored complement activation's role in oral cancer progression. The novel multiplex assay demonstrated specific detection capabilities for C3a, C5a, and sC5b-9 in minimal sample volumes from human tears, aqueous humor, and blood, with validated specificity and no cross-reactivity, enhancing its potential utility in OSCC biomarker identification.

FAQ

Related Products

As an expert deeply involved in complement research, Creative Biolabs has made amazing progress in complement testing. Hundreds of kits that detect multiple complement proteins simultaneously are the cornerstone of our complement testing services. For more complement multiplex assay service details, please contact us in time.

References

1. Kuhn, Amelie, et al. "Terminal complement activation is induced by factors released from endplate tissue of disc degeneration patients and stimulates expression of catabolic enzymes in annulus Fibrosus cells." Cells 12.6 (2023): 887.
2. Gallenkamp, Juliane, et al. "A novel multiplex detection array revealed systemic complement activation in oral squamous cell carcinoma." Oncotarget 9.3 (2017): 3001-3013.
3. Distributed under Open Access license CC BY 4.0, without modification.

• Complement Function/Activity Test
• Complement Autoantibody Test
• Complement Genetic Test